河南省新疆出血热血清流行病学调查  

Seroepidemiological Investigation on CCHF in Henan Province

作　　者：李林红[1] 张彦平[1] 吴振溢[1] 郝宗宇[1] 唐青[2] 陶晓霞[2] 西条政幸 

机构地区：[1]河南省疾病预防控制中心 人兽共患病防治研究室,郑州450003 [2]中国疾病预防控制中心传染病预防控制所 [3]日本国立感染症研究所

出　　处：《中国预防医学杂志》2005年第5期426-428,共3页Chinese Preventive Medicine

摘　　要：目的调查河南省新疆出血热的感染情况。方法采集不同地区牧羊人、一般农民、发热病人、既往EHF患者及家属的人血清标本及动物血清标本作横断面调查,采用基于昆虫细胞表达的CCHFV核蛋白建立的ELISA法对血清标本进行初筛,阳性及可疑阳性再用表达CCHFV核蛋白的HeLa细胞进行IFA试验加以确证。结果254份人血清及242份羊血清标本全部为阴性。结论调查表明河南省尚未发现新疆出血热病毒感染。应进一步调查,加强监测,做好防范新疆出血热的准备,尤其预防新疆出血热的输入感染。Objective To investigate the attack rate of CCHF in Henan province. Methods Sere samples from shepherds, veterinarians, slaughter workers, fever cases and animal hosts of CCHFV were collected in different areas of Henan province, positive samples were screened primarily by ELISA based on recombinant Baculovirus expressed CCHFV- NP and then confirmed by IFA which was based on CCHFC - NP expressing HeLa cells. Results All sere samples were negative for CCHF virus antibody. Conclusion The investigation results showed that no CCHFV infection was found in both human and animals in Henan province, This suggested that the surveillance of CCHF should be strengthened, and necessary measures should be adopted to prevent the possible occurrence of CCHF.

关 键 词：出血热 克里米亚 血清流行病学 输入病例 

分 类 号：R181.3[医药卫生—流行病学]