绿竹不同栽培类型RAPD分子标记的研究  

Cultivar Classification of Dendrocalamopsis oldhami with RAPD Molecular Markers

作　　者：余学军[1] 张立钦[1] 方伟[1] 杨萍[1] 

机构地区：[1]浙江林学院林业与生物技术学院,浙江临安311300

出　　处：《西南林学院学报》2005年第4期98-101,共4页Journal of Southwest Forestry College

基　　金：浙江省科技厅资助项目(2004C320056)

摘　　要：利用RAPD标记对22个不同栽培类型的绿竹进行多态性分析.从粗筛后的94个10 bp随机引物中筛选出20个有效引物,共扩增出113条DNA带,其中91条为多态性带,占总数的80.5%.不同引物扩增出不同的DNA指纹图谱.利用POPGEN32进行聚类分析,结果表明:聚类结果可将绿竹的不同栽培类型区分开来;这些不同栽培类型绿竹的遗传距离为0.122 2～0.815 4,大部分在0.6以下;RAPD分子标记对竹类植物种下等级进行分类有一定的意义.Polymorphic analyses on 22 cultivars of Dendrocalamopsis oldhami were made by means of RAPD technique. 20 effective primers with 10 bp were screened out of 94 primers, totally 113 DNA loci were amplified,and 91 of these loci were polymorphic （ accounting for 80. 5% ）. The UPGMA cluster analysis of POPGEN32 program showed the following results：The cultivars of Dendrocalamopsis oldhami could be classified by UPGMA analysis. The genetic distance among the studied cultivars was between 0. 122 2 and 0. 815 4,and most of the genetic distance was below 0.6. The technique of RAPD molecular markers was helpful to the under species classification of bamboo plants.

关 键 词：绿竹 RAPD 分子标记 多态性 栽培类型 

分 类 号：S795.501[农业科学—林木遗传育种]