库尔勒香梨砧木组织培养  

Study on Proliferation in Vitro of Dwarfing Interstock S and F series in Kuerle Fragrant Pear

作　　者：曾斌[1] 李疆[1] 张孝霖[1] 张强 史兰花 郭玉成 

机构地区：[1]新疆农业大学园艺学院校级重点植物组织培养实验室,新疆乌鲁木齐830052 [2]库尔勒香梨股份有限公司,新疆库尔勒841000

出　　处：《经济林研究》2006年第2期41-43,共3页Non-wood Forest Research

基　　金：新疆维吾尔自治区科技厅"库尔勒香梨产业化研究"项目中的子课题"优质香梨脱毒快繁技术研究"的研究内容。(编号20000101003)

摘　　要：试验选用库尔勒香梨矮化中间砧S系、F系中的品种嫩茎尖作为试验材料进行了离体增殖研究,结果表明,不同种类的基本培养基及不同浓度配比的植物生长调节剂对试管苗增殖的效果不同。M S培养基较A S、B5培养基更有利于矮化砧S系、F系茎的增殖,中等浓度的细胞分裂素(BA)与较低浓度的生长素(IBA)、赤霉素(GA3)配合使用效果更理想。综合茎质量、茎数量等指标认为:M S+BA 1.0 m g/L+IBA 0.05 m g/L+GA31.0 m g/L是矮化砧S系、F系试管苗茎增殖的最佳培养基。Taken dwarfing interstock S and F series in Kuerle fragrant pear as materials, tender stem tips were cultured in vitro. The results showed that the effects of kinds and concentrations of plant growth regular on proliferation in vitro were different. MS medium was more suitable for proliferation of stems than AS and B5 media, otherwise ~ combination of BA of medium concentration, GA3and IBA of lower concentration could have an ideal result. MS medium supplemented with BA （1.0 mg/L）, IBA （0. 05 mg/L） and GA3 （1.0 mg/L） was optimum for proliferation and growth of shoots, comprehensively considering the indexes, for examPle, quality and quantity of stems.

关 键 词：库尔勒香梨 矮化中间砧S系 F系 离体增殖 

分 类 号：S661.2[农业科学—果树学]