小尾寒羊催乳素基因5′侧翼调控区的克隆与序列分析  

Cloning and sequence analysis of 5′ flanking region of prolactin gene in Small Tail Han sheep

作　　者：王训翠[1] 储明星[2] 陈宏权[3] 朱国祺[1] 方丽[2] 叶素成[2] 

机构地区：[1]安徽中医学院科研处,合肥230038 [2]中国农业科学院畜牧研究所,北京100094 [3]安徽农业大学动物科技学院,合肥230036

出　　处：《安徽农业大学学报》2006年第3期314-317,共4页Journal of Anhui Agricultural University

基　　金：科技部科技基础条件平台工作项目(2004DKA30450)资助

摘　　要：采用PCR技术扩增出小尾寒羊催乳素基因5′侧翼调控区的161 bp大小的片段,经SSCP检测到2种基因型(AA、AB),将这2种基因型片段分别克隆到pGEM-T Easy质粒中,重组质粒用PCR扩增进行阳性克隆鉴定,然后测定核苷酸序列。催乳素基因扩增片段第63处发生了单碱基的改变(C→A)。小尾寒羊催乳素基因2种基因型核苷酸序列与母牛的同源性为92.5%。The 161 bp fragment of 5＇flanking region of prolactin gene of Small Tail Han sheep was amplified successfully by PCR. Two genotypes （ AA and AB） were detected by SSCP analysis and cloned into pGEM-T Easy vector. The positive clones were further identified by PCR amplification. The sequencing results showed that there was one single nucleotide mutation C---~A at the site of 63 bp of amplified fragment of prolactin gene. The homology of nucleotide sequence of 5 ＇flanking region of prolactin gene between Small Tail Han sheep and cow was 92.5%.

关 键 词：小尾寒羊 催乳素基因 克隆 序列分析 

分 类 号：S826.2[农业科学—畜牧学]