Nafion膜修饰无分离步骤的IgM电化学免疫传感器研究  

A Separation-free Amperometric Immunosensor for the Determination of IgM Based on Nafion Modified Electrode

作　　者：周亚民[1] 王永东[1] 王桦[2] 吴朝阳[2] 沈国励[2] 

机构地区：[1]东莞理工学院化学生物工程系,广东东莞523106 [2]湖南大学化学生物传感与计量学国家重点实验室,湖南长沙410082

出　　处：《分析测试学报》2006年第4期20-23,共4页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(20375008);广东省教育厅自然科学研究项目(Z03093);东莞市科技局项目(ZC060401ZC051209)

摘　　要：发展了一种基于Nation膜修饰电极构制的无分离步骤的异相免疫分析法，羊抗人IgM抗血清和邻氨基酚通过Nation膜固定在电极表面，形成生物敏感膜。采用竞争吸附免疫分析法，以HRP—IgM作标记物，只有结合在生物敏感膜的酶标物才能催化邻氨基酚与H2O2的反应，产生电分析信号，从而与游离在溶液里的酶标物区分开。因此分析过程勿需洗脱步骤，简化了分析过程，在优化的条件下，对IgM的检测范围为0．07～1．8mg／L。该分析技术操作简单、分析速度快。A separation-free electrochemical heterogeneous immunoassay based on the Nation membrane modified electrode has been developed for the determination of IgM. The anti-IgM antiserum and the o-amino phenol were immobilized on the electrode surface forming a biological sensitive membrane. Using the competitive adsorption immunoassay, and HRP - IgM as the label, it was found that only the enzyme label bound on the biological sensitive film could catalyze the reaction between o-amino phenol and H2O2 to generate amperometric signal. Thus, no washing step for separation of free and bound enzyme labels was required and the assay procedure was simplified. Under optimized conditions, the linear range of the calibration curve for IgM was in the range of 0.07 to 1. 8 mg/L. The developed assay is simple and rapid.

关 键 词：无分离步骤 免疫传感器 Nation膜修饰电极 IGM 

分 类 号：O657.15[理学—分析化学]