石英芯片电泳分离检测尿中蛋白的研究  

Analysis of Proteins in Urine by Quartz Chip Electrophoresis

作　　者：宋宏伟[1] 王惠民[1] 丛辉[1] 金庆辉[2] 贾春平[2] 刘菁[2] 庄贵生[2] 孙承龙[1] 赵建龙[2] 

机构地区：[1]南通大学附属医院,江苏南通226001 [2]中国科学院上海微系统与信息技术研究所,上海200050

出　　处：《分析测试学报》2006年第4期32-35,共4页Journal of Instrumental Analysis

基　　金：国家"863"重大专项项目(2002AA404310;2004AA404252);江苏省卫生厅重大项目(H200216)

摘　　要：通过对缓冲体系、缓冲液浓度、酸度、乳酸钙浓度、乙胺浓度、电泳电压和进样时间的优化选择，用石英芯片电泳一紫外检测法分离了纯人白蛋白和人运铁蛋白；在75mmol／L硼酸盐（pH10．55）（含0．8mmol／L乳酸钙、1％（φ）乙胺）运行缓冲液中，上述两组分在3min内完全分离；纯人白蛋白和人运铁蛋白的线性范围分别为1．0～15．0g/L和1．0～10．0g／L；检出限（S／N=3）均为0．5g／L，应用于临床尿蛋白分离测定，并与Helena琼脂糖凝胶电泳仪电泳结果进行比较，获得一致结果。Purified human albumin and human transferrin were successfully separated by quartz chip electrophoresis and detected by UV detection with addition of calcium lactate and ethylamine in borate buffer system. The effects of buffer, concentration and pH of the buffer, concentration of calcium lactale and ethylamine, separation voltage and injection time were investigated in this paper. Satisfactory separation of purified human albumin and human transferrin was achieved in 75 mmol/L borate buffer containing 0. 8 mmol/L calcium lactate and 1% （φ） ethylamine as running buffer（pH 10. 55 ） within 3 min. The linearities of the calibration curves were in the range of 1.0 - 15.0 g/L for purified human albumin and 1.0 - 10.0 g/L for human transferrin with the same detection limits （ S/N = 3 ） of 0. 5 g/L. This method was successfully used for separation and determination of clinical urine proteins. The resuhs of this assay agreed with those of Helena agarose gel electrophoresis.

关 键 词：石英芯片电泳 紫外检测 乳酸钙 乙胺 尿蛋白 尿 纯人白蛋白 人运铁蛋白 

分 类 号：O657.1[理学—分析化学] Q51[理学—化学]