基于I^--N-氯代丁二酰亚胺-鲁米诺反应体系流动注射化学发光法测定白藜芦醇  

Flow Injection Chemiluminescence Method for the Determination of Resveratrol with I^--N-chlorosuccinimide-Luminol System

作　　者：姚型军[1] 王术皓[1] 杜凌云[1] 

机构地区：[1]聊城大学化学化工学院,山东聊城252059

出　　处：《分析测试学报》2006年第4期88-91,共4页Journal of Instrumental Analysis

基　　金：山东省中青年学术骨干与学科带头人培养基金资助

摘　　要：基于在碱性介质中,白藜芦醇对I-增强N-氯代丁二酰亚胺-鲁米诺化学发光体系的阻抑作用,建立了测定白藜芦醇的流动注射化学发光分析新方法。优化了流动注射化学发光的分析条件和探讨了反应的可能机理。测定白藜芦醇的线性响应质量浓度范围为2.5×10-9~8.0×10-8g.mL-1,检出限为6.3×10-10g.mL-1,用5.0×10-8g.mL-1的白藜芦醇标准溶液进行11次测定,相对标准偏差(RSD)为2.2%。应用本法测定了虎杖、葡萄酒、葡萄皮样品中的白藜芦醇含量,结果满意。A novel flow injection chemiluminescence method has been developed for the determination of resveratrol based on its inhibition of the chemiluminescence from the iodide - N-chlororsuccinimide - luminol system in alkaline medium. The possible mechanism of the chemiluminesce reaction and the optimum analytical operating conditions have been studied. The linear range of resveratrol determination is 2.5×10^-9～8.0×10^-8g·mL^-1 with a detection limit of 6.3×10^10g·mL^-1. The relative standard deviation（RSD） （n = 11 ） is 2.2% for 5.0×10～g·mL^-1 resveratrol. The method has been applied to determine the content of resveratrol in grape skin, grape wine and traditional Chinese medicine polygonumcuspidatum seib with satisfactory results.

关 键 词：N-氯代丁二酰亚胺 鲁米诺 化学发光 白藜芦醇 流动注射 

分 类 号：O657.39[理学—分析化学] O625.31[理学—化学]