多重PCR同时检测口蹄疫病毒、猪水疱病病毒和水疱性口炎病毒  

Simultaneous Detection of FMDV,SVDV and VSV by Multiplex Polymerase Chain Reaction

作　　者：钟金栋[1] 花群义[2] 肖荣海[2] 夏雪山[1] 杨云庆[2] 周晓黎[2] 董俊[2] 邓祖洪 

机构地区：[1]昆明理工大学生化学院,云南昆明650224 [2]云南出入境检验检疫局技术中心,云南昆明650228 [3]西双版纳兽医站,云南景洪666100

出　　处：《动物医学进展》2006年第7期55-58,93,共5页Progress In Veterinary Medicine

基　　金：国家"十五"重大科技攻关项目(2001BA804A482004BA519A40)

摘　　要：根据基因库中的口蹄疫病毒(FM-DV),猪水疱病病毒(SVDV)和水疱性口炎病毒(VSV)各基因序列,设计了与FMDV,SVDV和VSV互补的3对特异性引物,对样品中的cDNA模板进行了多重PCR扩增及反应条件的优化,结果同时得到与设计相符合的3条特异性条带,分别为189 bp,125 bp和300 bp。用这3对引物对病毒样品cDNA模板进行多次扩增,均能稳定得到与设计相符合的3条特异性条带。本试验能特异、敏感、快速地鉴定FMDV,SVDV和VSV。A multiplex polymerase chain reaction was developed and optimized to detect Foot-and-moutn disease virus （FMDV）, Swine vesicular disease virus （SVDV） and Vesicular stomatitis virus （VSV）. Three pairs of specific primers were designed according to the conservation sequences of FMDV,SVDV and VSV in GenBank. All samples containing FMDV, SVDV and VSV were amplified desirablly by the multiplex PCR using the three sets of primers and then the multiplex PCR conditions were optimized. Three expected specific bands of FMDV gene 189 bp, SVDV gene 125 bp and VSV gene 300 bp in length were amplified. The multiplex PCR method is rapid, sensitive and specific for detecting FMDV,SVDV and VSV.

关 键 词：口蹄疫病毒 猪水疱病病毒 水疱性 口炎病毒 多重PCR 

分 类 号：S855.3[农业科学—临床兽医学] S858.28[农业科学—兽医学]