枣离体叶片高效再生植株的研究  

Establishment of High-efficient in Vitro Leaf Regeneration System in Chinese Jujube

作　　者：周瑞金[1] 刘孟军[1] 

机构地区：[1]河北农业大学中国枣研究中心,河北保定071001

出　　处：《园艺学报》2006年第3期625-628,共4页Acta Horticulturae Sinica

基　　金：国家科技攻关项目(2001BA502B09-04);河北省科技攻关项目(04220111D);河北农业大学科技将帅计划项目;河北农业大学科学发展基金项目

摘　　要：以‘黄骅冬枣’组培苗叶片为试材,研究了叶片幼嫩程度、叶片来源、组培苗状态以及植物生长调节剂等对离体叶片诱导不定芽再生的影响,并获得了完整的再生植株。结果表明,以未生根组培苗中上部叶片再生效果较好;TDZ诱导叶片再生不定芽的效果显著优于BA;离体叶片在MS+TDZ1·0mg·L-1+IBA0·1mg·L-1培养基中诱导培养28d后,转入MS+IBA0·1mg·L-1+GA30·05mg·L-1培养基中二次培养,叶片再生效果最好,再生率可达92·45%。将叶片再生植株转入MS+BA1·0mg·L-1+KT0·5mg·L-1+IBA0·1mg·L-1培养基中继代增殖培养,增殖系数达3·64。以1/2MS+IAA1·0mg·L-1培养基诱导生根,生根率87·1%。生根苗大田移栽成活率达到57%。Factors affecting in vitro leaf regeneration including age of leaf, source of leaf, condition of tube-plantlet and hormones in culture medium were studied in Ziziphus jujuba ‘Huanghua Dongzao＇. A highefficient system of in vitro leaf regeneration was established. The upper and middle leaves from rootless tube-plantlet were easier to regenerate than those from rooted tube-plantlet. The efficiency of TDZ was significantly higher than BA in the induction of adventitious bud from leaf. Leaves should be first induced on MS medium supplemented with TDZ （ 1.0 mg · L^-1 ） and IBA （0. 1 mg · L^-1） for 28 days, and than transferred to medium MS ＋ IBA 0. 1 mg · L^-1 ＋ GA3 0. 05 mg · L^-1. In this way, the regeneration rate reached 92.45%. MS ＋ BA 1.0 mg · L^-1 ＋ KT 0. 5 mg · L^-1 ＋ IBA 0. 1 mg · L^-1 was suitable for subculture of shoots, with the multiplication coefficient of 3.64. The regenerated plantlets rooted well in 1/2MS medium plus IAA （ 1.0 mg · L^-1 ）, with rooting percentage of 87.1%. The survival rate of rooted plantlets in the field was 57%.

关 键 词：枣 叶片培养 植株再生 

分 类 号：S665.1[农业科学—果树学]