农杆菌介导的百合遗传转化体系的建立  

Establishment of Agrobacterium-mediated Transformation System of Lily

作　　者：王爱菊[1] 张凤美[1] 鹿金颖[1] 赵祥云[2] 贾旭[1] 刘敏[1] 

机构地区：[1]中国科学院遗传与发育生物学研究所生物技术重点实验室,中国科学院研究生院北京100101 [2]北京农学院园林系,北京102206

出　　处：《园艺学报》2006年第3期664-666,共3页Acta Horticulturae Sinica

摘　　要：通过根癌农杆菌介导的遗传转化方法,建立了凝望星空百合（Lilium‘Star Gazer’）愈伤组织和西伯利亚百合（Lilum‘Siberia’）叶片的遗传转化体系.试验结果表明：以愈伤组织和叶片为受体,均能取得较理想的转化效果;根癌农杆菌OD600介于0.5～1.0时,能获得较高的转化率;40 mg·L^-1的潮霉素对愈伤组织和叶片有很好的筛选效果;在共培养培养基中,去除NH4NO3对提高百合转化效率有极显著的效果.对转基因百合进行PCR检测,结果表明gus基因已整合到百合基因组中.We established genetic transformation system of lily callus （ Lilium ‘Star Gazer＇ ） and leaves （Lilium ‘Siberia＇ ） mediated by Agrobacterium tumefaciens. Experimental result indicates that we could get efficient transformation with both callus and leaves as acceptor. We gained high transformation rate when the OD600 of Agrobacterium was between 0. 5 - 1.0. There were better screening to callus and leaves when the concentration of Hygromycin was 40 mg · L^-1. Removing NH4NO3 from major element could remarkably enhance transformation rate. Results of PCR proved that the gus gene had integrated into lily genome.

关 键 词：百合 根癌农杆菌 遗传转化 PCR 

分 类 号：S682.2[农业科学—观赏园艺]