竞争抑制法检测抗-HBc总抗体的影响因素及对策  

Influential factors in the detection of total anti-HBc antibody by competitive enzyme-linked immunosorbent assay

作　　者：吴淑梅[1] 林云[1] 王辉[1] 唐平[1] 沈茜[1] 

机构地区：[1]第二军医大学附属长海医院实验诊断科,上海200433

出　　处：《检验医学》2006年第4期376-379,共4页Laboratory Medicine

摘　　要：目的研究酶联免疫吸附试验(ELISA)待测血清标本与辣根过氧化物酶(HRP)标记的乙型肝炎病毒(HBV)核心抗体(抗-HBc-HRP)加入间隔时间对抗-HBc总抗体检测结果的影响。方法选择HBV标志物检测结果为阴性的标本分A、B、C 3组:A组,加样后延长室温放置不同时间再加入抗-HBc-HRP;B组,加样同时加入抗-HBc-HRP延长室温放置不同时间;C组,A组方式测定的阴性转阳性标本再用ELISA试剂2和化学发光(CLIA)试剂重复测定,确认阴、阳性结果。结果A组加样方式对抗-HBc总抗体检测结果影响明显,且加样和加入抗-HBc-HRP的间隔时间越长,标本的假阳性越多;B组加样方式对抗-HBc总抗体检测结果基本无影响;C组ELISA试剂1和试剂2有72.8%的检测结果一致;20例ELISA阴性转阳性标本经过CLIA方法验证仍为阴性。结论A组的不公平竞争在不同时间内可出现程度不等的抗-HBc总抗体假阳性;B组加样方式可最大限度地减少抗-HBc总抗体假阳性,保证检验质量。Objective To investigate the effect of interval between the addition of serum sample and anti-HBc-horseradish peroxidase（HRP） conjugate on the detection result of total anti-HBc antibody. Methods Serum anti-HBc was detected by domestic enzyme-linked immunosorbent assay （ELISA） kits and by Abbott chemiluminescent immunoassay （CLIA） system as control method. 75 pretested anti-HBc negative samples were divided into three groups. Group A： anti-HBc-HRP was added after the addition of serum sample and deposited at room temperature for different time（unfair competition） ; Group B：the serum sample and anti-HBc-HRP were added simultaneously and then deposited at room temperature for different time （ fair competition ） ; Group C ： the positive samples in group A were detected by another domestic ELISA reagent according to the same intervals and tested finally by abbott CLIA method. Results The pattern of addition of serum sample and anti-HBc-HRP affected the real results significantly. Furthermore, the longer interwls between the two additions led to more false-oositive results. Whereas. simultaneous addition of serum and anti-HBc-HRP had no effects on the real results. In group C, the results of 72.8 percentage of positive samples were consistent with group A. Twenty positive samples in group A showed negative reaction by abbott CLIA method. Conclusions The interval between the addition of serum sample and anti-HBc-HRP could lead to false positive result, which can be avoided maximumly with simultaneous addition,

关 键 词：酶联免疫吸附试验 乙型肝炎病毒核心抗体 假阳性 

分 类 号：R446.61[医药卫生—诊断学]