临床分离多重耐药铜绿假单胞菌β-内酰胺酶基因分析  

A genetic analysis of β-lactamase produced by multidrug-resistant Psedomonas aeruginosa separated from clinical specimens

作　　者：张珏[1] 靡祖煌[2] 乔昀[1] 

机构地区：[1]上海中医药大学附属曙光医院检验科,上海200021 [2]无锡市克隆遗传技术研究所,江苏无锡214026

出　　处：《检验医学》2006年第4期409-412,共4页Laboratory Medicine

摘　　要：目的 了解多重耐药铜绿假单胞菌β-内酰胺酶基因的存在情况。方法 收集2004年11月～2005年4月从临床七分离的17株多重耐药铜绿假单胞菌进行6种β-内酰胺酶基因的检测。结果 17株菌中检出TEM基因为8株（47．1％），VIM、OprD2基因均为2株（11．8％），而IMP、SHV、OXA基因均为阴性。结论 我院多重耐药铜绿假单胞菌的β-内酰胺酶基因主要是TEM基因，其次为VIM基因，OprD2缺失阳性率低，提示铜绿假单胞菌对亚胺培南耐药的机制不是单一的。Objective To find out the existence of the β-lactamase gene produced by muhidrug-resistant Psedomonas aeruginosa （P. aeruginosa）. Methods Six β-lactamase genes produced by 17 strains of muhidrug resistant P. aeruginosa separated from clinical specimens during Nov 2004 to Apr 2005 were detected. Results Of 17 strains, 8 showed a TEM gene （47, 1%）, 2 showed a VIM gene （11.8%） and 2 showed an OprD2gene （11.8%）. IMP, SHVand OXA genes were negative. Conclusions TEM is the major gene and VIM is the next place of the β-lactamase produced by muhidrug-resistant P. aeruginosa in our hospital. The absence of OprD2 gene has lower positive rate, which shows the absence of EMP-D2 is not the only mechanism of the drug-resistance of P. aeruginosa to imipenem.

关 键 词：铜绿假单胞菌 多重耐药 Β-内酰胺酶 基因 

分 类 号：R378.99[医药卫生—病原生物学]