小花鬼针草中咖啡酰奎宁酸类成分及其抑制组胺释放活性(英文)  

Caffeoylquinic acid derivatives from Bidens parviflora and their antihistamine release activites

作　　者：王珏[1] 王乃利[2] 姚新生[2] 北中进[1] 

机构地区：[1]日本大学药学部生药学研究室 [2]沈阳药科大学天然药化教研室,辽宁沈阳110016

出　　处：《中草药》2006年第7期966-970,共5页Chinese Traditional and Herbal Drugs

摘　　要：目的研究小花鬼针草B id ens p arv if lora全株的化学成分,并通过抑制组胺释放活性方法寻找生物活性化合物。方法采用硅胶、Sephadex LH-20和ODS柱色谱分离化合物,运用1D NM R,2D NM R等波谱法鉴定了化学结构,通过组胺抑制实验探讨抗炎活性。结果分离鉴定6种咖啡酰奎宁酸类化合物及其甲酯,分别是3,5-二氧咖啡酰奎宁酸(3,5-d i-O-caffeoy lqu in ic ac id,Ⅰ)、3,4-二氧咖啡酰奎宁酸(3,4-d i-O-caffeoy lqu in ic ac id,Ⅱ)、4,5-二氧咖啡酰奎宁酸(4,5-d i-O-caffeoy lqu in ic ac id,Ⅲ)、4-氧-咖啡酰奎宁酸(4-O-caffeoy lqu in ic ac id,Ⅳ)、5-氧-咖啡酰奎宁酸(5-O-caffeoy lqu in ic ac id,Ⅴ)、4-[3-(3,4-二羟基苯基)-丙烯酰氧基]-2,3-二羟基-2-甲基-丁酸{4-[3-(3,4-d ihydroxy-pheny l)-acry loy loxy]-2,3-d ihydroxy-2-m ethy l-bu tyric ac id,Ⅵ}。结论所有化合物均为首次从该植物中分得,化合物Ⅵ为新化合物。这些化合物显示一定的抑制组织胺释放活性。Objective Based on the activities of antihistamine release to study the compounds from Bidens parvi-flora and find biological active compounds. Methods The chemical constituents from B. parviflora were isolated by silica gel and Sphadex LH-20 column chromatographies and purified by preparative HPLC. The chemical structures had been identified by physiochemical properties and spectroscopic methods. Results Six caffeoylquinic acid derivatives were identified as 3, 5-di-O-caffeoylquinic acid （Ⅰ ）, 3, 4-di-O-caffeoylquinic acid （ i ）, 4, 5-di-O-caffeoylquinic acid （ Ⅱ）, 4-O-caffeoylquinic acid （ Ⅳ ）, 5-O- caffeoylquinic acid （Ⅴ ）, 4-[- 3-（ 3, 4-dihydroxy-phenyl ）-acryloyloxy]-2, 3-dihydroxy-2-methyl-butyric acid （ Ⅵ）. Conclusion Compounds Ⅰ～Ⅵ are first obtained from B. parviflora and Ⅵ is new one. Some of the compounds exhibit the activities in antiallergic assays. Moreover, the structure-activity relationships of these compounds have been also discussed in this paper.

关 键 词：小花鬼针草 咖啡酰奎宁酸 组胺抑制活性 

分 类 号：R284.1[医药卫生—中药学]