大孔吸附树脂纯化红花提取物的研究  

Purification of Carthamus tinctorius extract with macroporous adsorption resins

作　　者：宋洪涛[1] 初阳[2] 张倩[2] 郭涛[3] 陈大为[2] 李可鑫[1] 刘伟[1] 颜鸣[3] 

机构地区：[1]南京军区福州总医院药学科,福建福州350025 [2]沈阳药科大学药学院,辽宁沈阳110016 [3]沈阳军区总医院药剂科,辽宁沈阳110016

出　　处：《中草药》2006年第7期996-1000,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(30200363);辽宁省博士启动基金资助项目(2001102042)

摘　　要：目的探讨大孔吸附树脂对红花提取物的纯化条件及纯化效果。方法通过红花黄色素(SY)在树脂上的吸附量和解吸率筛选树脂的种类;以SY的保留率和转移率为指标考察药液的pH值、上柱吸附体积流量、树脂药材比、树脂柱径高比、清洗液的pH值及体积流量、洗脱液的种类及体积流量等纯化条件。与乙醇沉淀法进行纯化效果的比较。结果优化后的纯化条件为采用HPD 400A作为吸附树脂,树脂药材比为4∶1,树脂柱径高比为1∶6,上柱药液和清洗液pH值为2.0,50%乙醇作为洗脱液,上柱吸附体积流量为4 BV/h,清洗和洗脱体积流量为10BV/h。在该纯化条件下,SY的转移率为88.8%,与乙醇沉淀法相当,固形物中SY的质量分数约为乙醇沉淀法的2.6倍,固形物的质量(固形物得率)仅为乙醇沉淀法的38.5%。结论通过纯化条件的优化大大提高了SY的转移率,大孔吸附树脂法对红花提取物的纯化效果优于乙醇沉淀法。Objective To study the conditions and effects of purification of Cartharnus tinctorius extract by adopting macroporuos adsorption resin. Methods The kinds of resin were selected through the determination of adsorption ratio and elution ratio of safflower yellow （SY） in resin. The reservation ratio and transfering ratio of SY were used as index to investigate the purification conditions, such as the pH value of water extract, the flow speed for adsorption, the ratio of the resin to crude drug, the ratio of diameter to height of resin column, the pH value and flow speed of cleaning agent, and the kinds and flow speed of the elution agent, etc. The purification results of macroporuos adsorption resin were compared with the alcohol sedimentation. Results The optimum purification conditions were as follows： HPD400A Macroporuos adsorption resin was selected as the adsorbed resin, the ratio of resin to crude drug was 4 ： 1, the ratio of diameter to height of resin column was 1 ： 6, the pH value of water extract and cleaning agent was adjusted to 2.0, 50% alcohol was chosen as elution agent, the flow speed of adsorption was 4 BV/h, the flow speed of cleaning and elution agent transfering ratio of SY was 88.8%, approximated to was 10 BV/h. Under the purification condition, the that by the alcohol sedimentation method. Compared with the alcohol sedimentation method, the macroporous adsorption resin purification method could increase the SY content in dried solid about 2.6 times, while be the weight of dried solid about 38.5%. Conclusion The transfering ratio of SY is greatly improved through the optimization of the purification conditions. The method of macroporous adsorption resin purification is superior to the method of alcohol sedimentation.

关 键 词：红花 红花黄色素 大孔吸附树脂 纯化 

分 类 号：R284.2[医药卫生—中药学] R286.02[医药卫生—中医学]