四种生物碱逆转肿瘤获得性多药耐药与调控细胞凋亡的相关性研究  

Reversal effect of 4 alkaloids on apoptosis of being obtained multi-drug resistance to tumour cell

作　　者：李贵海[1] 潘成业 孙付军[1] 王学荣[1] 尹格平[3] 

机构地区：[1]山东省中医药研究院,山东济南250014 [2]山东烟台市毓璜顶医院,山东烟台256001 [3]济南军区总医院,山东济南250031

出　　处：《中成药》2006年第7期1001-1004,共4页Chinese Traditional Patent Medicine

基　　金：山东省自然基金资助项目编号:Y2002C37

摘　　要：目的:观察四种中药生物碱对模拟临床化疗PFC方案诱导的小鼠S180细胞多药耐药基因mdr1表达蛋白P170过度表达的逆转与调控细胞凋亡相关性,探讨不同中药生物碱对肿瘤多药耐药逆转的差异,以指导临床应用,提高化疗疗效。方法:给予模拟临床PFC方案以亚于治疗剂量的联合化疗诱导四周的腹水型S180小鼠,苦参碱、粉防己碱、氧化苦参碱和盐酸小檗碱2周,流式细胞仪免疫荧光检测S180肿瘤细胞P170、TOPOⅡ、Fas、Trail和细胞凋亡率。结果:苦参碱、粉防己碱明显降低化疗诱导后耐药细胞P170表达率和TOPOⅡ活性,增加凋亡基因Fas表达率,促进耐药肿瘤细胞的凋亡;盐酸小檗碱明显降低化疗诱导后耐药细胞TOPOⅡ的表达率。结论:苦参碱、粉防己碱逆转肿瘤细胞获得性多药耐药P170过度表达与调控细胞凋亡有关。AIM： To observe the relationship between the reversal effect of 4 alkaloids of TCM on expression of P170 being obtained multi-drug resistance to mouse S180 tumor cell induced by chemotherapy protocol of PFC and the adjustment of the cell apoptosis. And to discuss the difference among alkaloids of TCM on reversion of being obtained multi-drug resistance in clinic. To improve the curative effect thereby. METHODS： By the methods of less dosage of Chemotherapy PFC,to set up the mouse models of multi-drug resistance of S180 tumor cell. At the same time to give the mouse matrine, terandrine, oxymatrine and berberine hydrooh loride for 2 weeks and then to observe the P170,TOPOⅡ , Fas and apoposis by flow cytometry. RESULTS： Matrine and terandrine could obviously reduce the expression of P170,the activation of TOPO Ⅱ induced by Chemotherapy and increase the ratio of the express of Fas and the apopsis of drug resistance to tumor cell. And berberine hydrochloride could obviously reduce the expression of TOPO Ⅱ. CONCLUSION ： The adjustment to the apoposis of tumor cell by matrine and terandrine is related to the reversion of being obtained multi-drug resistance to tumor cell＇ expression, besides the different degree

关 键 词：苦参碱 粉防己碱 氧化苦参碱 盐酸小檗碱 获得性多药耐药 小鼠 P170 拓扑异构酶Ⅱ Fas 

分 类 号：R285.6[医药卫生—中药学]