光学分子成像技术观察纳流芯片对DNA分子的研究(英文)  

Manipulating DNA Molecules in Nanofluidic Channels and Observing with Optical Molecular Imaging

作　　者：王凯歌[1] 岳双林[2] 王雷[3] 金爱子[2] 顾长志[2] 王鹏业[4] 徐祥福[1] 李冀[1] 牛憨笨[1] 

机构地区：[1]深圳大学光电子学研究所,深圳518060 [2]中国科学院物理研究所微加工实验室,北京100080 [3]西安应用光学研究所光电子部,西安710065 [4]中国科学院物理研究所软物质实验室,北京100080

出　　处：《光子学报》2006年第7期1056-1060,共5页Acta Photonica Sinica

基　　金：Supported by the National Natural Science Foundation ofChina ( No . 60025516 , No . 10334100 , and No .60138010 );the Innovation Project of the ChineseAcademy of Sciences ,K.C.Wong Post-doctoral ResearchAward ( No .40 );partly supported by NationalCenter for Nanoscience and Technology

摘　　要：将λ-DNA与荧光染料YOYO-1结合,利用荧光显微技术对DNA分子在毛细现象作用下进入宽40 nm、深60 nm的纳米沟道内,并在其内部被拉伸以及沿沟道移动的情形进行了观察.讨论了DNA分子在沟道内的运动情况.结合光学分子成像技术与该尺寸范围的纳流芯片,将有助于研究生物分子的动力学和静力学性质.λ-phage DNA molecules, stained with YOYO-1 fluorescent dye, were put inside sub- 100 nm open channels which have width～40 nm and depth ～60 nm. The DNA molecules were transported along these nanoconduits and the removing information was observed by fluorescence microscopy technique clearly. The movements of DNA molecules in the channels were discussed. Associating with optical molecular imaging, these scale nanostructured fluidic channels would be useful for studying single biomolecules statics as well as the dynamics.

关 键 词：光学分子成像 DNA分子 纳流芯片 纳米孔 

分 类 号：Q63[生物学—生物物理学]