高效液相色谱法测定清热利胆合剂中黄芩苷的含量  

Determination of Baicalin in Qingrelidan Heji by HPLC

作　　者：宋振民[1] 

机构地区：[1]山东省聊城市人民医院,252000

出　　处：《时珍国医国药》2006年第7期1192-1193,共2页Lishizhen Medicine and Materia Medica Research

摘　　要：目的建立测定清热利胆合剂中黄芩苷的高效液相色谱（HPLC）测定方法。方法黄芩苷测定以kromasil ODS C18（4．6mm×200mm，5μm）为固定相，甲醇-水-磷酸（53：47：0．2，WWV）为流动相，UV检测波长为280nm。结果黄芩苷在0．20—1．80μg范围内与峰面积呈直线关系。回归方程为：Y=251481．75X-6859．95，r=0．9999，平均回收率为99．66％，RSD=1．14％。结论该法测定清热利胆合剂中黄芩苷的含量简便、准确、快捷，可用于清热利胆合剂的质量控制。Objective To establish HPLC method for baicalin in Qingrelidan Heji . Methods Baicalin was separated on kromasil ODS C18 （4.6 mm × 200 mm ,5 μm） column and detected at 280 nm, with methanol-water-phosphoric acid （53： 47： 0.2, V/V/ V） as mobile phase. Results There was good linearity from 0.20 to 1.80 μg （ Y = 251 481.75X - 6 859.95, r = 0. 999 9 ） in separation of baicalin. The average recovery was 99.66% with RSD of 1.14%. Conclusion This method is a simple ,rapid, and accurate. This study can be as the reference to establish quality standard for Qingrelidan Heji.

关 键 词：高效液相色谱法 黄芩苷 清热利胆合剂 

分 类 号：R284.2[医药卫生—中药学]