短小棒状杆菌纯化物对小鼠骨髓瘤细胞的作用  

Anti-tumor and immunological effect of the purified corynebacterium parvum on the myeloma cell of mice

作　　者：吴隼[1] 韩效林[1] 王琬玲[1] 

机构地区：[1]新乡医学院第一附属医院血液科,河南卫辉453100

出　　处：《新乡医学院学报》2006年第4期346-348,共3页Journal of Xinxiang Medical University

摘　　要：目的初步探求短小棒状杆菌(CP)纯化物是否具有抗肿瘤作用及其免疫机理。方法短小棒状杆菌纯化物(酶解法)及短小棒状杆菌分别注入负瘤小鼠。氚标记胸腺嘧啶掺入法(3H-TdR)测小鼠脾淋巴细胞转化试验;流式细胞仪对T淋巴细胞亚群进行检测;mRNA表达法检测细胞因子TNF和INF-γ的活性。结果短小棒状杆菌纯化物(酶解法)及CP均可抑制肿瘤细胞生长。免疫功能显示,CP和酶解法纯化的CP都能刺激机体T淋巴细胞的转化(P<0.05)、CD3+、CD4+、CD8+数值显著增加及细胞因子的表达增强(P<0.05),且酶解法纯化的CP还能刺激机体CD8+值显著增加(P<0.05)。结论酶解法纯化短小棒状杆菌不仅抑制骨髓瘤细胞生长,并能显著的激活巨噬细胞系统,刺激机体的细胞免疫功能。Objective To study the tumor suppression end the immune enhancement effect of perified corynebacterium parvum（CP） in the myeloma cell of mice end the probable mechanism. Methods The entitumor effect of the CP end CP pefification on the myeloma cell was determined in the BABL/C mice; spleen lymphocyte cells transformation was tested by 3H- TdR methods. The number of T clusters was analyzed by the flow cytometer. And cellular immune response was evaluated by mRNA of TNF and INF - 7. Results CP end the purification of CP could inhibit the growth of tumor cells, decrease the tumor weight end increase the spleen weight（P〈 0.05） ; increase the number of CD^3 ＋, CD^4 ＋ , CD^8 ＋ end the ratio of CD^4 ＋ end CD^8 ＋ cells in the spleen （ P 〈 0.05） ; But could not increase the number of NK cells. Con. clusion The CP and the purification of CP could significantly inhibit the growth of tumor in vivo, end enhancing the immunological function may be one of the reasons for the anti - tumor mechanism.

关 键 词：短小棒状杆菌 抗肿瘤 纯化 CD4^+细胞 CD8^+细胞 

分 类 号：R551.3[医药卫生—血液循环系统疾病]