1，6－二磷酸果糖对心肌细胞胞质游离Ca^（2＋）和pH的影响  

EFFECTS OF FRUCTOSE-1,6-DIPHOSPHATE(FDP) ON INTRACELLULAR Ca￣(2+) AND pH IN ISOLATED MYOCYTES

作　　者：王宏[1] 江文德[1] 

机构地区：[1]上海医科大学基础医学院药理学教研室

出　　处：《上海医科大学学报》1996年第6期423-426,共4页Journal of Fudan University(Medical Science)

摘　　要：考察外源性的１，６－二磷酸果糖（ＦＤＰ）对心肌细胞内游离Ｃａ２＋浓度和ｐＨ值的影响。用荧光探针（Ｆｕｒａ－２，ＢＣＥＣＦ）技术同时测定成年大鼠心肌细胞内游离Ｃａ２＋浓度和ｐＨ值，并观察其在缺氧／复氧条件下的变化。结果：在缺氧／复氧过程中，胞质游离Ｃａ２＋浓度缓慢升高，从基础值１３０±２９．５ｎｍｏｌ／Ｌ１升至７４２±１５４ｎｍｏｌ／Ｌ，胞质ｐＨ值则从７．１２±０．０８降至６．７１±０．１３。在含有５ｍｍｏｌ／ＬＦＤＰ的细胞悬液中，则胞质游离Ｃａ２＋上升幅度明显低于对照组（Ｐ＜０．０５），在复氧结束时的最高值为５３８±１９３ｎｍｏｌ／Ｌ，但胞质ｐＨ值的变化与对照组比较无显著差别。结论：ＦＤＰ可以减轻由缺氧／复氧所造成的心肌细胞内游离Ｃａ２＋浓度增高的程度，而对胞质ｐＨ值的变化无明显影响。PURPOSE To study if exogenous fructose-1,6-diphosphate (FDP)exerts homeostatic regulatory effect on intracellular Ca2+ ([Ca2+]i) elevation and pH ([pH]i) changes during hypoxia/reoxygenation in isolated rat myocytes.METHODS Utilizing fluorescent Ca2+and H+ indicators (Fura-2 and BCECF)techniques to study the effect of FDP on disturbances of [Ca2+]i and [pH]i in isolated rat cardiocytes simultaneously which were exposed to hypoxia succeeded by reoxygenation.RESULTS Exposure to hypoxia followed by re-oxygen produced a sustained increases in [Ca2+]i from 130±29.5 nmol/L upto 742±154 nmol/L,and a slow decrease in [pH]i from 7.12± 0.08 down to 6.71± 0.13.FDP (5 mmol/L) significantly attenuated the characteristic damage reflected by stepping-up increase in [Ca2+]i (538±193 nmol/ L,P<0.05), but did not prevent the occurrence of intracellular acidosis (6.74±0.18, P>0.05). CONCLUSIONS FDP prevents the increase in [Ca2+]i level in myocytes isolated from rat heart during hypoxia/reoxygenation, but unaccompanied by correction of acidosis.

关 键 词：二磷酸果糖 心肌细胞 游离CA^2+ 细胞质 pH值 

分 类 号：R977.6[医药卫生—药品]