高效液相色谱法测定乳病消片中橙皮苷的含量  

Content Determination of Hesperidin in Rubingxiao Tablet by HPLC

作　　者：李红卫[1] 焦海胜[1] 李平[1] 张花[2] 

机构地区：[1]兰州大学第二医院,兰州市730030 [2]兰州大学药学院,兰州市730000

出　　处：《中国药房》2006年第14期1105-1106,共2页China Pharmacy

摘　　要：目的建立以高效液相色谱法测定乳病消片中橙皮苷含量的方法。方法色谱柱为ZorbaxSBC18,流动相为乙腈-冰醋酸-水(21∶1∶78),流速为1.0ml/min,检测波长为283nm,柱温为40℃,进样量为20μl。结果橙皮苷进样量在0.0328μg^0.328μg范围内与峰面积积分值线性关系良好(r=0.9998),平均加样回收率为98.81%(RSD=0.69%)。结论本方法准确、简便、重现性好,可用于本品的质量控制。OBJECTIVE： To establish a HPLC method for determination of the content of hesperidin in Rubingxiao tablets.METHODS： Zorbax SB C18 column was used as chromatographic column, CH3CN- CH3COOH- H2O）（21 ： 1 ： 78） as mobile phase .The flow rate was set at 1.0ml/min, and the detection wavelength at 283nm.The column temperature was 40℃, and the sample size was 20μl.RESULTS： The calibration curve of hesperidin was linear within the range of 0.0 328μg- 0. 328μg（ r = 0.9 998） .The average recovery was 98.81% （RSD = 0.69% ） . CONCLUSION： The established method is accurate,convenient as well as reproducible,and is applicable for the quality control of hesperidin in rubingxiao tablets.

关 键 词：高效液相色谱法 乳病消片 橙皮苷 含量 

分 类 号：R927.2[医药卫生—药学]