藻类基因转录调控研究进展  

Progress in Transcriptional Control of Algae Genes

作　　者：魏炜[1] 孟春晓[2] 秦松[1] 

机构地区：[1]中国科学院海洋研究所 [2]山东理工大学生命科学院,山东淄博255049

出　　处：《海洋通报》2006年第4期78-84,共7页Marine Science Bulletin

基　　金：国家自然科学基金(No.30470156)资助

摘　　要：转录调控是基因表达调控的重要方式。转录调控的研究主要集中在启动子的克隆与分析、反式作用因子(即DNA结合蛋白)的检测及转录量的检测三方面。介绍了基因组步移、凝胶阻滞、酵母双杂交系统、cDNA微阵列等基因转录调控的常用研究方法,并对藻类基因转录调控的最新研究进展进行了评述。Transcriptional control is an important way of regulating of gene expression. It is studied mainly in three fields： cloning and analysis of promoter region, finding of DNA-binding protein and activity of gene transcription. In this paper, the widely used techniques for the study of transcriptional control, such as genome walking, electrophoresis mobility shift assay, yeast two-hybrid system, cDNA microarray and so on are introduced and the recent progress in the transcriptional control of algae gene is discussed.

关 键 词：转录调控 顺式作用元件 反式作用因子 启动子 DNA结合蛋白 藻类 

分 类 号：Q786[生物学—分子生物学]