Fe^（2＋）存在时H_2O_2对鸟嘌呤的氧化作用  

OXIDATIVE EFFECT OF HYDROGEN PEROXIDE ON GUANINE IN THE PRESENCE OF FERROUS IONS

作　　者：宋元宗[1] 庄海旗[1] 张海涛[1] 祝其锋[1] 莫丽儿[1] 

机构地区：[1]广东医学院医用生化研究所,广东医学院化学教研室

出　　处：《广东医学院学报》1996年第4期312-314,共3页Journal of Guangdong Medical College

摘　　要：用毛细管柱气相色谱／质谱分析法（ＣＧＣ／ＭＳ）研究了Ｆｅ^（２＋）存在时Ｈ_２Ｏ_２对鸟嘌呤的氧化作用。结果表明：Ｈ_２Ｏ_２或Ｆｅ^（２＋）单独应用均不能氧化鸟嘌呤（Ｐ＞０．０５），而两者联合应用却可以氧化鸟嘌呤而生成８－羟基鸟嘌呤（Ｐ＜０．０１，其含量约为对照组的５０倍）。结论：Ｈ_２Ｏ_２的对鸟嘌呤的氧化作用可能是通过·ＯＨ介导的，而不是Ｈ_２Ｏ_２自身的直接作用。The oxidative effect of H_2O_2 on guanine in the presence of Fe￣(2+) was studied by means of capillary gas chromatography/mass spectrometry(CGC/MS). The results showed that neither H_2O_2 nor Fe￣(2+)could oxidize guanine(P<0.05),while H_2O_2 in the presence of Fe￣(2+) resulted in the formation of 8-hydroxyguanine(P<0. 01,about 50 times as control). It is suggested that the oxidative effect of H_2O_2 on guanine be mediated by·OH,while not results from its direct effect.

关 键 词：质谱分析法 鸟嘌呤 H2O2 CGC/MS 

分 类 号：Q526.2[生物学—生物化学]