北五味子及其不同炮制品中五味子甲素和乙素的含量测定  

Determination of content of deoxyschizandrin and-schizandrin in the different processed productss of Fructus Schisandrae Chinensis by HPLC

作　　者：魏东华[1] 宋智敏[1] 陈淑欣[1] 刘金成[1] 

机构地区：[1]哈尔滨医科大学大庆校区,黑龙江大庆163319

出　　处：《现代生物医学进展》2006年第6期20-21,共2页Progress in Modern Biomedicine

摘　　要：目的：测定北五味子及其不同炮制品中五味子甲素、五味子乙素的含量。方法：采用高效液相色谱法。Spherisorb 10 ODS1（4、6mm×250mm，10μm），以甲醇-水（70：30）为流动相，检测波长为254nm。线性范围0．1-0．5μg，相关系数为O．995．平均回收率为100．3％和100．4％，RSD=0．87％和2．85％（n=5）。结果：五味子甲素和乙素在生品、醋炙、炒制、酒炙、蒸炙、蜜炙品中的平均含量分别为0．13％，0．064％，0．052％，0．064％，0．11％，0．13％和O．21％，0．298％，0．238％，0．302％，0．216％，0．214％。结论：方法准确，快速，重现性好，可用于五味子各种炮制品的质量评价。Objective： To compare the content of deoxyschizandrin and γ- schizandrin in the raw medical material and the different processd products of Fructus Schisandrae Chinensis（FSC）. Methods： The content of deoxyschizandrin and γ- schizandfinwere was determined by HPLC（ = high performance liquid chnanatography）, with Sphefisorb 10 ODS1 （4.6mm× 250mm, 10μm）, the mobile phase of a mixture of methanol- water（70：30）, and detecting wave length of 254nm. The standard curve was linear in the range of 0. 11-0.55μg, with correlation coefficient 0.995. The average recovery Of FSC was 100.3% and 100.4%, with RSD=0.87% and 2.85%. Results： The mean content of dcoxyschizandrin was 0.13%, 0.064%, 0.052%, 0.064%, 0.11%, 0.13% and γ- sehizandrin, 0.21%, 0.298%, 0.238%, 0.302%, 0.216%, 0.214%, in the different processed products of FSC. Conlusion：The method is accurate, quick and good reappearanee, which can be used for the quality evaluation in the different processed products of FSC.

关 键 词：北五味子 炮制 高效液相色谱法 五味子甲素 五味子乙素 含量测定 

分 类 号：TQ461[化学工程—制药化工] S567.79[农业科学—中草药栽培]