HSP90以及IL-6在系统型红斑狼疮发病中的作用  

The Role of Heat Shock Protein 90 and Interleukin-6 in the Pathogenesis of Systemic Lupus Erythematosus

作　　者：徐卿[1] 胡绍先[1] 黄钢红 吴记平[1] 杨柳[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院风湿科,武汉市430030 [2]武钢总医院风湿科,武汉市430080

出　　处：《医学分子生物学杂志》2006年第4期268-270,共3页Journal of Medical Molecular Biology

摘　　要：目的研究系统型红斑狼疮(systemiclupuserythematosus,SLE)患者外周血单个核细胞(PBMC)中热休克蛋白90(HSP90)、血清中白细胞介素6(IL6)的水平,探讨与活动期SLE的相关性。方法应用Western印迹检测20例SLE活动期患者(A组)与20例正常人(B组)PBMC中HSP90的表达,并利用酶联免疫法(ELISA)检测其血清中IL6水平。结果①A组HSP90表达水平为0.47±0.05,B组为0.18±0.07,差异有统计学意义(P<0.01);②血清中IL6水平在A组[(32.97±8.65)pg/ml]显著高于B组[(10.46±4.33)pg/ml](P<0.05);③A组血清中IL6水平与PBMC中HSP90表达呈正相关(r=0.61,P<0.01),且与SLE疾病活动指数(SLEDAI)正相关(r=0.48,P<0.01)。结论IL6可作为病情活动监测指标,IL6与HSP90的异常高表达可能在SLE发病机制中起重要作用。Objective To investigate the expression of heat shock protein 90 （HSP90） in peripheral blood mononuclear cells （PBMC） and the serum level of interleukin-6 （IL-6） in the active systemic lupus erythematosus （ SLE）, and to explore their relation with the active SLE. Methods Western blot technique was used to analyze HSP90 expression in peripheral blood mononuclear cells （ PBMC ） and ELISA was applied to determine the serum level of IL-6 from 20 SLE patients （ group A） and 20 normal controls （ group B） . Results ① the mean level of HSP90 expression in group A and group B were 0. 47 ± 0. 05 and 0. 18 ± 0. 07, respectively. Comparison showed that, the mean level of HSP90 expression in group A was significantly increased （ P 〈 0. 01 ） ; ② the mean serum levels of IL-6 in group A and group B were 32. 97 ±8.65 pg/ml and 10. 46 ±4.33pg/ml, respectively. The difference between the two group was significant （ P 〈 0. 05 ） ; ③there was a positive correlation between the level of IL-6 and HSP90 in group A （ r = 0. 61, P 〈 0. 01 ） , and the level of IL-6 and SLEDAI had a positive correlation in group A （ r = 0. 48, P 〈 0. 01 ） . Conclusion The results suggest that the serum level of IL-6 might be used as one of the indicators for active SLE. Their abnormal expression may play an important role in the pathogenesis of SLE.

关 键 词：系统型红斑狼疮 热休克蛋白90 白细胞介素-6 

分 类 号：R593.241[医药卫生—内科学]