D301阴离子交换树脂对大豆异黄酮分离特性的影响  

Separation of the Soybean Isoflavones by D301 Anion Exchange Resin

作　　者：田冠峰 徐明雅[2] 杨亦文[2] 魏作君[2] 吴彩娟[2] 任其龙[2] 

机构地区：[1]黑龙江九三油脂有限责任公司,哈尔滨150069 [2]浙江大学二次资源化工国家专业实验室,杭州310027

出　　处：《食品与发酵工业》2006年第6期133-136,共4页Food and Fermentation Industries

摘　　要：为更好地将离子交换树脂应用于大豆异黄酮的分离纯化,研究了乙醇含量、pH、温度、初始浓度等条件对D301阴离子交换树脂吸附分离大豆异黄酮的影响。静态吸附实验结果表明,该树脂在以水为溶剂、pH 8左右、40℃时对大豆异黄酮有最好的吸附效果。穿透曲线实验表明,动态饱和吸附量受温度影响较大,当柱温为50℃、初始浓度C07.03 mg/mL时,饱和吸附量为105.0 mg/g干树脂。洗脱曲线实验表明,采用体积分数75%乙醇洗脱,异黄酮回收率为89.3%,产品中大豆异黄酮含量高达56.0%,含量比原料提高了十几倍。表明D301阴离子交换树脂对大豆异黄酮的分离纯化具有较好的选择性和应用前景。In order to apply ion exchange resin to static as well as dynamic adsorption characteristics of the separation and purification of the soybean isoflavones, the the soybean isoflavones onto D301 anion exchange resin were studied. The optimum pH and temperature for static adsorption were pH 8 and 40℃, respectively. Breakthrough curves showed that the adsorption quantity was affected by temperature, and when the temperature was 50＊（2 and the initial concentration was 7.03 mg/mL, the saturated adsorption quantity of isoflavones on D301 resin was 105. 0 mg/g. Eluting the isoflavones from the resin using 75% （V/V） ethanol as the solvent, the product with the content of 56.0% soybean isoflavones was obtained. The results showed that D301 anion exchange resin was a good adsorbent for the separation of soybean isoflavones from soybean extract.

关 键 词：大豆异黄酮 D301阴离子交换树脂 吸附等温线 穿透曲线 洗脱曲线 

分 类 号：TQ28[化学工程—有机化工]