借对乙酰基偶氮羧p-铕(Ⅲ)络合物作光度探针测定生物样品中蛋白质  

Eu(Ⅲ)-p-ACETYLCARBOXYLAZO-p AS PHOTOMETRIC PROBE FOR DETERMINATION OF PROTEIN IN BIOLOGICAL SAMPLES

作　　者：衷明华[1] 

机构地区：[1]韩山师范学院化学系,潮州521041

出　　处：《理化检验（化学分册）》2006年第7期560-561,564,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：江西省自然科学基金项目(0320039)

摘　　要：在pH3．0的Britton-Robinson缓冲介质中，蛋白质能与铕（Ⅲ）-对乙酰基偶氮羧p形成复合物，最大吸收峰波长为695nm，比络合物探针本身红移145nm。可用于生物样品中蛋白质的测定，牛血清白蛋白（BSA）的线性范围为0．010～0．030g·L^-1，相关系数为0．9954；人血清白蛋白（HAS）的线性范围为0．005～0．025g·L^-1，相关系数为0．9947，应用于测定生物试样中的总蛋白质，RSD小于6．5％，回收率在98．4％～103．7％。In a B-R buffer medium of pH 3. 0, a colored compound complex was formed between protein and the binary complex of Eu（ Ⅲ ）-p-Acetylcarboxylazo-p （abbreviated as Eu（ Ⅲ ）-pA-p）, having its absorption maxima at the wavelength of 695 urn, with a bathochromic shift of 145 um from the absorption maxima of Eu（ Ⅲ ）-pA-p complex. Based on this color reaction, the binary complex, Eu（ Ill ）-pA-p, was used as a photometric probe for the determination of protein in biological samples. Calibration curves for determination of BSA and HAS were prepared and linear relationships between the absorbancy values and concentrations of protein were obtained as follows., for BAS 0. 010-0. 030 g · L^-1 （r=0. 995 4） and for HAS 0. 005-0. 025 g · L^-1 （r=0. 994 7）. In the determination of total protein in two biological samples, RSD＇s （n=5） of the results obtained were less than 6. 5%, and recoveries were in the range of 98. 4%-103. 7%.

关 键 词：蛋白质 铕(Ⅲ)-对乙酰基偶氮羧p络合物 光度探针 生物样品 

分 类 号：O65[理学—分析化学]