雄性糖尿病大鼠海马神经元凋亡及其相关蛋白表达的改变  

Changes of apoptosis and its related protein expressions of hippocampal neurons in male rats with diabetes mellitus

作　　者：赵红光[1] 徐松柏[2] 吕喆[1] 郭伟[1] 李艳博[1] 赵刚[1] 龚守良[1] 

机构地区：[1]吉林大学公共卫生学院卫生部放射生物学重点实验室,吉林长春130021 [2]吉林大学第一医院神经外科,吉林长春130021

出　　处：《吉林大学学报（医学版）》2006年第4期550-553,F0002,共5页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(39870312)

摘　　要：目的:观察糖尿病(DM)大鼠海马神经元凋亡及其周期和相关蛋白的改变。方法:链脲佐菌素(STZ)腹腔注射诱导DM大鼠模型,12周末通过流式细胞术检测其海马神经元凋亡,碘化丙啶(PI)单染检测其周期,罗丹明123染色检测其线粒体膜电位,免疫组化法检测其细胞色素c(Cyt c)和caspase-3蛋白表达。结果:DM大鼠出现明显的多饮、多食、多尿及体重减轻现象。给药12周末,与正常对照组相比,其海马神经元凋亡率显著增加(P<0.05),G0/G1期细胞百分率显著增高(P<0.01),即出现明显的G0/G1期阻滞,S期细胞显著减少(P<0.05);线粒体膜电位显著增高(P<0.05);caspase-3和Cyt c蛋白表达均显著增高(P<0.05)。结论:上述参数的改变可能与DM所致胰岛素缺乏相关,进而引起海马结构的改变和认知功能的障碍。Objective To explore the changes of hippocampal neuron apoptosis, cell cycle progression and its related protein expressions in rats with diabetes mellitus （DM）. Methods The male rats were injected intraperitoneally with streptozosin （STZ） to establish DM models. At the end of 12th week, the percentage of the hippocampal neuron apoptosis in the rats suffered from DM was measured with flow cytometry （FCM）, and their cell cycle progression was measured with propidium iodide （PI） dying, and their mitochondrial membrane potential was measured with FCM under the Rhodamine 123 dying. The protein expressions of Cyt c and caspase-3 were detected with immunohistochemical methods. Results The diabetic rats developed obviously polydipsia, polyphagia, polyuria and loss of body weight. As compared with the normal controls, the cell percentage of the hippocampal neuron apoptosis in the diabetic rats at the end of the 12th week increased significantly （P〈0.05）; the cell percentage of G0/G1 phase increased significantly （P〈0. 01）, while the cell percentage of S phase decreased significantly （P〈0. 05）; the mitochondrial membrane potential increased significantly （P 〈 0.05）, and the expressions of caspase-3 and Cyt c proteins also increased significantly （P〈0. 05）. Conclusion The changes of indices mentioned above may be related to the absence of insulin in diabetic rats caused by hyperglycemia, which might be the reason for the change of hippocampus structure and cognitive function disorder in diabetic rats.

关 键 词：糖尿病 实验性 海马 神经元 细胞凋亡 细胞周期 

分 类 号：Q255[生物学—细胞生物学] R-332[医药卫生]