苯和甲醛对雄性小鼠睾丸生殖细胞的联合毒性  

Toxicity of benzene combined with formaldehyde on reproductive cells in mice

作　　者：李玲[1] 权铁刚[2] 于光艳[1] 李铁骥[1] 

机构地区：[1]吉林大学公共卫生学院环境与劳动卫生教研室,吉林长春130021 [2]吉林大学中日联谊医院急诊科,吉林长春130033

出　　处：《吉林大学学报（医学版）》2006年第4期609-611,共3页Journal of Jilin University：Medicine Edition

基　　金：吉林省卫生厅基金资助课题(200025)

摘　　要：目的:探讨苯和甲醛对雄性小鼠睾丸生殖细胞的毒性作用。方法:选用纯系雄性小鼠为研究对象,苯加甲醛的联合染毒剂量(mg.kg-1)分别为10+10、50+50、100+100(作为3个实验组),同时设对照组(0 mg.kg-1组)和植物油组(n=10)。连续经口染毒8周后处死小鼠,进行睾丸称重、精子计数、精子畸形百分率计算和睾丸组织超氧化物歧化酶(SOD)活力测定。结果:各染毒组小鼠的精子数随苯和甲醛联合染毒剂量的增加而逐渐减少,精子畸形率明显增加,与对照组比较差异有显著性(P<0.05,P<0.01);各染毒组小鼠睾丸组织SOD活力随苯和甲醛联合染毒剂量的增加而逐渐降低,当联合染毒剂量为(50+50)和(100+100)mg.kg-1时,与对照组比较差异有显著性(P<0.05,P<0.01);睾丸重量有随染毒剂量的增加而降低的趋势(P>0.05)。结论:苯和甲醛能对小鼠的生殖细胞产生明显联合毒性作用。Objective To study the toxicity of benzene combining with formaldehyde on reproductive cells in mice. Methods Male mice were randomly divided into different groups： three experimental groups with different doses of benzene combining with formaldehyde （mg · kg^-1 ） （10 ＋ 10, 50 ＋ 50 and 100 ＋ 100）, control group and vegetable oil group （n= 10）. After 8 weeks by oral poison, the mice were killed, testis was weighed, sperm count was peformed, the rate of sperm deformation was counted and the SOD activity was measured. Results The quantities of sperm in various poison groups were obvious decreased and the rate of sperm deformation were obvious increased with dose increasing. Compared with control group, they both had obvious difference （P 〈 0.05, P 〈 0.01）. Activities of SOD in testis in experimental groups were obvious decreased with dose increasing. And when the doses of benzene combined with formaldehyde were （50＋ 50） and （100 ＋ 100） mg · kg^-1, there had obvious difference compared with control group （P〈0. 05, P〈0. 01）. Conclusion Benzene combined with formaldehyde can cause obvious toxicity in reproductive cells.

关 键 词：苯/毒性 甲醛/毒性 联合染毒 精子计数 精子畸形率 超氧化物歧化酶 

分 类 号：R321.1[医药卫生—人体解剖和组织胚胎学] R-332[医药卫生—基础医学]