人β_2糖蛋白1的重组表达及对抗磷脂抗体综合征患者血清诱导内皮细胞产生ICAM-1的影响  

Recombination of human β_2-glycoprotein-1 and its effect on expression of ICAM-1 in endothelial cell line induced by plasma from anti-phospholipid antibody syndrome patients

作　　者：付嘉[1] 方艳秋[1] 徐立[1] 李明辉[1] 刘桂英[1] 姜艳芳[1] 段秀梅[1] 刘力华[1] 许淑芬[1] 谭岩[1] 

机构地区：[1]吉林大学第一医院中心实验室,吉林长春130021

出　　处：《吉林大学学报（医学版）》2006年第4期654-657,共4页Journal of Jilin University：Medicine Edition

基　　金：教育部高等学校博士学科点专项基金资助课题(20040183066);吉林省科技厅资助课题(20030419);吉林省杰出青年基金资助课题(20050113);吉林大学青年基金资助课题(2005年)

摘　　要：目的:探讨重组人β2糖蛋白1(hrβ2-GP1)对抗磷脂抗体综合征(APS)患者血清诱导内皮细胞系产生细胞间黏附分子-1(ICAM-1)的影响。方法:应用pQE30表达载体表达hrβ2-GP1,Western blotting对重组蛋白进行鉴定;应用hrβ2-GP1处理前后的APS患者血清分别与内皮细胞株EA.hy926共孵育,细胞ELISA和RT-PCR方法分析处理前后EA.hy926表达ICAM-1的变化。结果:成功构建pQE30-hβ2-GP1,并对表达产物进行了纯化和鉴定,所获得的蛋白与预期的大小一致,并具有人β2-GP1的抗原性;rhβ2-GP1处理后的APS患者血清与处理前比较,与其孵育的内皮细胞系EA.hy926表达ICAM-1在蛋白和mRNA水平均明显降低,这种抑制作用随着rhβ2-GP1的浓度增加而逐渐增强。结论:抗β2糖蛋白1抗体(anti-β2GP1)促进内皮细胞系EA.hy926表达ICAM-1,hrβ2-GP1可中和此作用。Objectivo To investigate the effect of human recombinant β2-glycoprotein-1 （hrβ2-GP1） on expression of intercellular adhesion molecular-1 （ICAM-1） in endothelial cell line induced by plasma from anti-phospholipid antibody syndrome （APS） patients. Methods hrβ2-GP1 was expressed using pQE30 expression vector and characterized by Western blotting, hrβ2-GP1 treated and untreated plasma from APS patients were incubated with endothilial cell line EA. hy 926. The changes of ICAM-1 expressed by EA. hy 926 were analyzed using cell-ELISA and RT-PCR. Results PQE30-hβ2-GP1 was constructed, the exprssion products were purified and characterized. Compared with the untreated one, the membranous protein and mRNA expression of ICAM-1 in EA. hy926 of the hrβ2-GP1 treated plasma from APS patients were significantly decreased. Conclusion Anti-β2-GP1 antibody can elevate the expression of ICAM-1 in endothelial cell line EA. hy926, hrβ2-GP1 can neutralize this effect.

关 键 词：重组人β2糖蛋白1 抗磷脂抗体综合征 内皮细胞系 细胞间黏附分子-1 

分 类 号：R392[医药卫生—免疫学]