人参皂苷Rh_2对胶质瘤细胞的体外抑制作用  

Inhibitory effect of ginsenoside-Rh_2 on glioma cells cultivated in vitro

作　　者：王占峰[1] 罗毅男[1] 洪新雨[1] 鞠砚[1] 付双林[1] 郭海涛[1] 李叶[1] 韩海玲[2] 孙玉芹[2] 

机构地区：[1]吉林大学第一医院神经外科,吉林长春130021 [2]北华大学医学院附属医院心脑血管科,吉林吉林132011

出　　处：《吉林大学学报（医学版）》2006年第4期658-661,共4页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅资助课题(20010533);吉林省中医药管理局资助课题(吉中医0237)

摘　　要：目的:比较人参皂苷Rh2、榄香烯与阿霉素对胶质瘤细胞的体外抑制及凋亡诱导作用,以及药物杀伤的时效关系。方法:体外培养胶质瘤细胞,用MTT肿瘤体外药敏法比较人参皂苷Rh2、榄香烯与阿霉素对胶质瘤细胞系C6、SHg-44、U-251的抑制作用,并绘制细胞存活率-时间曲线。用流式细胞术检测3种药物作用后细胞凋亡情况。结果:3种药物对胶质瘤细胞系C6、SHg-44、U-251的抑制作用呈明显的剂量依赖性,随浓度的增加而增强。人参皂苷Rh2对胶质瘤细胞系的抑制作用明显高于榄香烯和阿霉素,与后两者比较差异有显著性(P<0.01)。3种药物对胶质瘤细胞的抑制作用均呈时间依赖关系,随时间延长而增强,且低浓度的人参皂苷Rh2短时间(12 h)就有生长抑制作用,而榄香烯和阿霉素的起效时间较缓慢。3种肿瘤细胞经低浓度的3种药物作用后均可出现凋亡,其中人参皂苷Rh2凋亡细胞比率明显高于其他两组,差异有显著性(P<0.01)。结论:人参皂苷Rh2对胶质瘤具有较好的生长抑制和凋亡诱导作用,起效时间早于榄香烯和阿霉素。人参皂苷Rh2良好的抑瘤效果和迅速的起效时间具有应用于胶质瘤治疗的潜在价值。Objective To compare the inhibitory effects of ginsenoside-Rh2, elemene and adriamycin （AMD） on glioma cells cultivated in vitro. Methods MTT was used to detect the inhibitory effects of ginsenoside-Rh2, elemene and AMD on glioma cell lines （C6, SHg-44, U-251）. The survival rate-time curves were established. The abilities of three drugs to induce apoptosis of three cells lines were determined by flow cytometry. Results The inhibitory effects of the three drugs on the growth of C6, SHg-44 and U-251 were dose-dependent and could be enhanced with dose increasing. The inhibitory effects of Rh2 was stronger than that of AMD and elemene on three glioma cell lines. The inhibitory concentration of Rh2 on three cell lines was significantly lower than that of AMD and elemene （P〈0. 01）, the inhibitory effects of three drugs demonstrated time- and dosedependency and could be enhanced with time prolonging. Rh2 exhibited growth inhibition on glioma ceils at low concentration in short time （12 h）, elemene and AMD took effect slowly, whlie Rh2 exhibited higher apoptotic rate than other two drugs （P〈0. 01）. Conclusion Ginsenoside-Rh2 can exhibite excellent activity of growth inhibition and apoptosis induction on glioma cells. It＇s recommend for further clinical study in glioma treatment.

关 键 词：神经胶质瘤 人参皂苷 榄香烯 阿霉素 细胞凋亡 

分 类 号：R282.71[医药卫生—中药学] R739.41[医药卫生—中医学]