长春花碱和秋水酰胺诱导TK6和TK6-E6细胞TK基因突变比较研究  

Comparison of TK gene mutation assay in TK6 and TK6-E6 cell induced by vinblastin and colcemid

作　　者：王亚男[1] 帅培强[2] 丁晓琴[1] 张立实[1] 

机构地区：[1]四川大学华西公共卫生学院营养与食品卫生学教研室,成都610041 [2]四川省出入境检验检疫局

出　　处：《卫生研究》2006年第4期409-411,共3页Journal of Hygiene Research

基　　金：国家自然科学基金资助(No.30471473);博士点基金资助(No.20020610036)

摘　　要：目的比较TK6和TK6-E6人类淋巴母细胞TK基因突变试验对2种纺锤体毒物——长春花碱(VBL)和秋水酰胺(CCM)检出情况的差异。方法用长春花碱和秋水酰胺处理TK6和TK6-E6细胞24小时,进行常规TK基因突变试验,检测细胞毒性及tk位点突变频率。结果随着长春花碱和秋水酰胺浓度的增加,TK6和TK6-E6细胞的相对存活率(RS%)和相对悬浮生长率(RSG%)均降低。相同剂量下,TK6-E6细胞的RS%和RSG%均比TK6高。长春花碱和秋水酰胺对TK6和TK6-E6细胞均有致突变性,TK6-E6细胞的诱发和自发突变频率高于TK6,而除了突变频率(MF)中CCM5·0ng/ml组和SC%中CCM0·625ng/ml组外,其它相同剂量下,TK6-E6的MF和慢生长集落百分比(SC%)均高于TK6细胞(P<0·05)。结论两种细胞都可用于TK基因突变试验,但由于TK6-E6所用细胞量少,倍增时间较短,故建议使用TK6-E6细胞。Objective To compare TK gene mutation assay in human lymphoblastoid cell lines TK6 and TK6-E6 induced by vinblastin and colcemid. Methods Regular TK gene mutation assays were experimented to detect cytotoxicity and mutation frequency at tk locus after TK6 and TK6-E6 human lymphoblastoid ceils were treated with vinblastin and colcemid for 24h. Results Relative survival （ RS % ） and Relative suspension growth （ RSG% ） of TK6 and TK6-E6 cells decreased when concentrations of vinblastin and colcemid increased. RS% and RSG% of TK6-E6 cells were higher than that of TK6 ceils at the same doses. TK6 and TK6-E6 cells both were induced by vinblastin and colcemid. The TK6-E6＇s nature mutation frequency, induced mutation frequency and percentage of slow growth mutant（SG% ）were lower than TK6＇ s at the same dose except group CCM 5.0ng/ml in MF and group CCM 0.625ng/ml in SC%. Conclusion Both TK6 and TK6-E6 cell lines can be used in TK gene mutation assay, but TK6-E6 cell line was recommended to be used in the assay for its less quantity and doubling time.

关 键 词：TK基因突变试验 TK6 TK6-E6 长春花碱 秋水酰胺 

分 类 号：Q754[生物学—分子生物学] R992[医药卫生—毒理学]