胡桐愈伤组织诱导条件初报  

A Preliminary Report on Callus Induction of Calophyllum inophyllum

作　　者：邵志芳 罗焕亮[2] 郭勇[3] 

机构地区：[1]深圳市莲花山公园,广东深圳518049 [2]深圳出入境检验检疫局,广东深圳518045 [3]华南理工大学生物工程学院,广东广州510641

出　　处：《林业科技》2006年第4期1-3,共3页Forestry Science & Technology

基　　金：广州市科技新星计划资助项目(2000-K-003-01)

摘　　要：为了通过植物细胞培养获取胡桐抗HIV的活性物质,首次报道胡桐愈伤组织的诱导条件。研究结果表明:胡桐幼苗的叶、茎段与胚根均可诱导出愈伤组织,其中以茎段的诱导率最高,达95.6%,而胚乳难以诱导愈伤组织。胡桐愈伤组织生长与培养基中无机盐含量有关,在B5、MS、White三种不同的基本培养基中,以B5最适于胡桐愈伤组织的诱导,MS次之,White最差。In order to obtain the anti - HIV substance in CalophyUum inophyllum by plant cell culture, the calli induced factors of C. inophyUum were firstly reported. The calli could be induced from young leaves, stem and radicles of C. inophyllurn seedling on MS medium but could not from endosperms. Of the explants above, the highest calli- inducing frequency with 95.6 % was observed from stem. The calli growth was in relation to the concentration of inorganic salts in the medium. The best basic medium for the calli growth was B5 following with MS and White.

关 键 词：胡桐 愈伤组织 外植体 诱导 

分 类 号：S792.43[农业科学—林木遗传育种]