生防菌对青枯雷尔氏菌强致病力和无致病力菌株生长竞争的影响  

Influences of the biocontrol strain ANTI-8098A, Bacillus cereus on the growth competition between the virulent and avirulent strains of Ralstonia solanacearum

作　　者：葛慈斌[1] 刘波[1] 林营志[1] 朱育菁[1] 肖荣凤[1] 

机构地区：[1]福建省农业科学院生物技术研究所,福州350003

出　　处：《植物保护学报》2006年第2期151-156,共6页Journal of Plant Protection

基　　金：国家"863"计划项目(2002AA244031-2);福建省科技厅重大项目(2000Z031);福建省计委项目(闽计农经[2002]48号)

摘　　要：采用共培养法研究了不同温度下青枯雷尔氏菌强致病力菌株F-01-V与无致病力菌株F-01-A之间的生长竞争、生防菌ANTI-8098A胞外物质对F-01-V和F-01-A生长的影响以及对F-01-V与F-01-A之间生长竞争关系的影响。结果表明,在15、20、25、30和35℃,菌株F-01-V、F-01-A单独静置培养48h后,F-01-V活菌数的增长率最高可达123.7%,F-01-A的活菌数下降,最大降幅为60.7%;当F-01-V与F-01-A等量混合培养时,F-01-V能继续增长,但48h后的增长率不如单独培养时的高,F-01-A的生长受到促进,使得这两个菌株的活菌数之比(F-01-V/F-01-A)与混合前的比值(0.97)相近;生防菌胞外物质对F-01-V有较强的抑制作用,抑制率与温度呈正相关,对F-01-A具有促进生长的作用,促长率也与温度呈正相关;生防菌胞外物质对F-01-V的抑制作用和对F-01-A的促进生长作用,导致在生防菌液、F-01-V与F-01-A共存的环境中,F-01-V与F-01-A之间的生长竞争向着有利于F-01-A的方向发展,使F-01-A成为优势菌株,特别是在30℃以上的温度下,F-01-V/F-01-A小于0.01,F-01-A的优势极为明显。The present paper dealt with the growth competition between the virulent strain F-01-V and avirulent strain F-01 -A of Ralstonia solanacearum, and the effects of the extracellular substances of bacterial-wilt-disease blocontrol strain ANTI-8098A, Bacillus cereus on the growths of strains F-01 -V and F-01 - A as well as influences of strain ANTI-8098A on the growth competitions at different temperatures. The results showed that the numbers of strain F-01-V increased with the largest increasing rate of 123.7%, that of strain F-01-A decreased with the largest decreasing rate of 60.7%, when they were separately maintained at temperatures 15, 20, 25, 30 and 35 ℃ for 48 h, respectively. While they were in mixing cultures of strain F-01 -V and F-O1 -A for 48 h, the growth of strain F-01 -V was inhibited and the growth of strain F-01-A was facilitated. The extracellular substances of strain ANTI-8098A could inhibit the growth of strain F-01-V, but it could facilitate the growth of strain F-01-A. There was a positive correlation between the inhibiting （or the facilitating） rate and temperature. When the mixed cultures were maintained at temperatures at 30℃ , the ratio values of F-01-V/F-01-A were less than 0. 01, which indicated the strain F-01-A were significantly dominated.

关 键 词：青枯雷尔氏菌 生防菌ANTI-8098A 无致病力菌株 生长竞争 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]