B型烟粉虱携带传播烟草曲茎病毒的能力  

Acquisition and transmission of Tobacco curly shoot virus by the B biotype of the whitefly, Bemisia tabaci

作　　者：纠敏[1] 周雪平[2] 刘树生[1] 

机构地区：[1]浙江大学昆虫科学研究所,杭州310029 [2]浙江大学生物技术研究所,杭州310029

出　　处：《植物保护学报》2006年第2期168-172,共5页Journal of Plant Protection

基　　金：国家重点基础研究发展规划"973"项目(2002CB111403)

摘　　要：B型烟粉虱近20年来入侵许多国家和地区,已成为重要的世界性作物害虫,其主要危害方式之一是传播双生病毒,造成作物病毒病大发生。应用PCR技术,研究了入侵我国的B型烟粉虱个体获取、存留及传播烟草曲茎病毒(Tobacco curly shoot virus,TbCSV)的能力。B型烟粉虱在感染TbCSV的普通烟毒株上饲毒30min时,就可在40%的个体内检测到TbCSVDNA,个体带毒率随饲毒时间延长而增加,饲毒12h后,带毒率达100%;TbCSVDNA在B型烟粉虱体内可存留10天左右,但不能终身存留;传毒处理植株的发病症状及PCR检测结果表明,B型烟粉虱是TbCSV的传播媒介。The B biotype of the whitefly, Bemisia tabaci（ Gennadius）, has invaded many countries and regions of the world in the last 20 years and now become one of the most important crop pests worldwide. One of the major ways causing damage by this pest is transmission of geminiviruses. In this study, the capacity for B biotype of the whitefly to acquire, retain and transmit Tobacco curly shoot virus （TbCSV） was investigated using polymerase chain reaction （PCR）. TbCSV DNA was detected in 40% of whiteflies that had fed for 30 min on TbCSV-infected tobacco plants. Percent acquisition of TbCSV by individual whiteflies increased with increase of acquisition time and reached 100% after 12 h. TbCSV DNA was retained in viruliferous whiteflies for about ten days, but not for the entire life of the vector. As shown by the symptoms of the infected plants following virus transmission and the result by PCR, the B biotype of the whitefly proves to be a vector of TbCSV.

关 键 词：B型烟粉虱 烟草曲茎病毒 病毒传播 

分 类 号：S433[农业科学—农业昆虫与害虫防治]