芥蓝的组织培养研究  

Study on Tissue Culture of Chinese Kale(Brassica alboglabra Bailey)

作　　者：周林[1] 曾国平[1] 章崇玲[2] 邓智泉[2] 

机构地区：[1]华南农业大学园艺学院 [2]华南农业大学生命科学学院

出　　处：《长江蔬菜》2006年第8期45-46,共2页Journal of Changjiang Vegetables

摘　　要：以尖叶中迟芥蓝为材料,取其带柄子叶和下胚轴进行组织培养,用不同浓度的6-BA和不同浓度的NAA处理,之后在生根培养基上分化根。结果表明:不同配方的培养基和不同浓度的6-BA显著影响芥蓝愈伤组织的增殖和不定芽的分化,最佳配比为:MS+3%蔗糖+0.8%琼脂+2mg/L6-BA+0.2mg/LNAA。不同浓度的NAA、不同外殖体之间对芥蓝组培芽分化的影响不显著,不同激素的不同浓度也不能显著地提高根的分化率,但含0.2mg/L6-BA的生根培养基较合适。The cotyledons with petiole and hypocotyls were In Vitro Cultured in the media supplemented with different concentrations of 6-BA and NAA ,in Chinese kale（Brassica alboglabra Bailey）. The results showed that different recipes and different concentrations of 6-BA could affect the multiplication of organ and the polarization of adventitious bud markedly, the best recipe of the culture medium was：MS ＋ 3% sucrose ＋ 0.8% agar＋2 mg/L 6-BA ＋0.2 mg/L NAA. The differences were not observed in organogenesis between different concentrations of NAA and different explants. The medium with 0.2 mg/L 6-BA was optium for shoot to root.

关 键 词：芥蓝 组织培养 再生体系 

分 类 号：S635.9[农业科学—蔬菜学]