以N-甲基葡胺为磷酸化受体测定血清碱性磷酸酶的实验探讨  

Experimental study on assay of serum alkaline phosphatase using N-methyl-D-glucamine buffer as phosphorylating acceptor

作　　者：蒋胜高[1] 王波[1] 王毓三[1] 赵建华[1] 

机构地区：[1]江苏省临床检验中心,南京210009

出　　处：《临床检验杂志》2006年第4期271-273,共3页Chinese Journal of Clinical Laboratory Science

摘　　要：目的评估以N-甲基葡胺作为磷酸化受体缓冲液测定血清碱性磷酸酶（ALP）活性的方法。方法通过对试剂的合理组合和实验条件的优选，建立基于N-甲基葡胺的血清ALP测定方法，并与基于其他缓冲液的推荐方法进行对比。结果精密度，批内：CV=0．62％，批间：CV=2．3％；线性范围至少可达到2073U／L；分别与以2-氨基-2-甲基-1．丙醇（AMP，X1）和二乙醇胺（DEA，X2）为磷酸化受体的ALP测定法比较：Y=1．29X1＋13．25，r=0．999；Y=0．65X2＋19．19，r=0．998。健康成人参考区间为44～133U／L[（80．33±20．97）U／L]。结论N-甲基葡胺可替代AMP或DEA作为磷酸化受体缓冲液用于临床血清ALP活性的常规测定。Objective To evaluate the method of measuring activity of serum alkaline phosphatase （ALP） using N-methyl-D-glucamine （MEG） buffer as phosphorylation acceptor. Methods A new method for measurement of serum ALP activity was established based on reasonable organization of reagent solutions and optimization of the reaction conditions, and it was compared with the recommended methods using other buffers. Results The intra-assay CV was 0.62% （ n = 20） for ALP activity of 333U/L and the inter-assay CV was 2.3% （ n = 20） for ALP activity of 180 U/L. The range of linearity was extended to at least 2 073 U/L. The ALP activity determined by the MEG-based assay correlated well with the results determined by AMP-based assay（ Y = 1.29X1 ＋ 13.25 ,r =0. 999） or DEA-based assay（ Y = 0.65X2 ＋ 19.19, r = 0.998 ）. The ALP reference values from healthy adults were 44 to 133 U/L with a mean value of 80.33 U/L. Conclusion MEG may be used as the phosphorylating acceptor buffer to substitute AMP or DEA for assay of serum ALP activity.

关 键 词：血清 碱性磷酸酶 N-甲基葡胺 

分 类 号：R446.1[医药卫生—诊断学]