快速酶联免疫吸附试验检测TORCH-IgM抗体的研究  

Fast ELISA for detection of TORCH-IgM antibodies.

作　　者：吴坚美[1] 朱波[1] 王红梅[2] 朱有名[1] 王志宇[1] 吴围屏[1] 邵金辉[1] 

机构地区：[1]山东省医药生物技术研究中心,山东济南250062 [2]山东中医药大学附属医院

出　　处：《山东医药》2006年第20期1-3,共3页Shandong Medical Journal

基　　金：山东省计划生育科学技术发展计划资助项目(鲁人口发[2005]70号)。

摘　　要：目的建立快速检测TORCH-IgM抗体的方法。方法利用聚乙二醇(PEG)能加速抗原、抗体反应的特点,在常规间接EL ISA法检测TORCH-IgM抗体基础上,在样本稀释液、酶标记物稀释液中各加入3%PEG,以缩短反应时间。结果温育时间缩短到15 m in(37℃)的快速EL ISA法对检测人TORCH-IgM抗体强阳性、弱阳性、阴性标本具有稳定性好,精密性、特异性强的特点;稀释液中不加PEG的常规15 m in法,采用37℃、15 m in试验条件,可能会造成弱阳性标本漏检情况。结论快速EL ISA法的建立为TORCH病原体感染的快速检测和流行病学调查提供了新的手段。[Objective] Establishing a fast method for detecting TORCH-IgM antibodies. [Methods] The PEG could accelerate the reaction between the antigen and the antibody. On the basement of the indirect ELISA method for detecting TORCH-IgM antibody, 3%PEG was added to the sample dilution liquid and the enzyme marker dilution liquid to shorten the reaction time. [Results] The new method was stable, accurate, and specific to detect strong positive, weak positive and negative TORCH-IgM antibodies strong positive, weak positive, and negative, using the new method, as the incubation time shortened to 15 min at 37℃. The weak positive samples might be failed to detect by the normal method 15′in dilution liquid without PEG when the incubation time shortened to 15 min at 37℃. [Conclusion] Fast ELISA provides a new method for fast detecting TORCH-IgM antibodies and for epidemiological study.

关 键 词：TORCH感染 免疫球蛋白M 聚乙二醇 酶联免疫吸附试验 

分 类 号：R181.3[医药卫生—流行病学] R715.2[医药卫生—公共卫生与预防医学]