离体肾脏保存过程中bcl-2蛋白、bcl-2mRNA表达变化及FK506对其的影响  

The change of bcl-2、bcl-2 mRNA expression in isolated renal preservation and the effect of FK506

作　　者：梁鲁南[1] 祝海洲[2] 赵伟[3] 肖林[1] 崔耕刚[3] 

机构地区：[1]济宁医学院附属医院,山东济宁272100 [2]济宁医学院 [3]济宁市第一人民医院

出　　处：《山东医药》2006年第20期7-8,共2页Shandong Medical Journal

基　　金：山东省教育厅立项资助课题(鲁科教鉴字[2005]第121号)

摘　　要：目的探讨离体肾脏保存过程中bcl-2蛋白、bcl-2 mRNA表达及他可莫司(FK 506)对其的影响。方法将40只W istar雄性大鼠随机分为实验组和对照组各20只,建立离体肾脏模型。对照组将离体肾脏置于常规4℃UW保存液中;实验组在上液中加入FK 506,分别检测肾脏保存2、4、8、16 h后bcl-2蛋白、bcl-2 mRNA表达。结果实验组4、8、16 h后bcl-2蛋白、bcl-2 mRNA表达明显高于对照组(P<0.05)。结论FK 506可能通过上调bcl-2蛋白、bcl-2 mRNA表达,减轻离体肾脏保存过程中的损伤。[Objective] To investigate bcl-2, bcl-2-mRNA expression in renal preservation and effect of FK506. [Methods] 40 male Wistar rats were randomly divided into test group and control group,the model of the preservation of isolated rat renal was established. The isolated rat renal was put into 4℃ UW liquid in the control group and mixed FK506 in the test group. Renal tissue bcl-2,, bcl-2 mRNA expression were studied respectively with immunohistochemical staining, reverse transcription polymerase chain reaction （PT-PCR） at different preservation time points （2,4,8,16 h）. [Result] The bcl-2, bcl-2 mRNA expression in test group were obviously increased （P〈 0. 05）. [Conclusion] FK506 may decrease the isolated rat renal injury through increasing bcl-2, bcl-2 mRNA expression.

关 键 词：他可莫司 肾脏 BCL-2蛋白 

分 类 号：R699.2[医药卫生—泌尿科学]