大别山区六种黄精属植物的五种同工酶分析  

Isoenzyme pattern analysis for five kinds of enzyme in six species of Polygonatum from Dabieshan Mountain

作　　者：陈存武[1] 周守标[2] 

机构地区：[1]皖西学院化学与生命科学系,安徽六安237012 [2]安徽师范大学生命科学院,安徽芜湖241000

出　　处：《广西植物》2006年第4期395-399,共5页Guihaia

基　　金：安徽省自然科学基金资助项目(00042415)~~

摘　　要：采用聚丙烯酰胺凝胶电泳法对皖西大别山区六种黄精属植物的抗坏血酸氧化酶(AOD)、多酚氧化酶(PPO)、超氧化物歧化酶(SOD)、酯酶(EST)和过氧化物酶(POD)五种同工酶进行了比较分析。结果表明:(1)五种同工酶共显示出66条酶带,其中有3条是黄精属的特征酶带;并且来源于不同种的同一种酶的谱带数、相对迁移率、酶活性均不相同,呈现多样性;(2)五种同工酶谱的模糊聚类分析结果与形态学分类的结果一致,利用酶谱差异可以将六种黄精初步区分;(3)在六种黄精属植物中,湖北黄精、轮叶黄精、多花黄精和金寨黄精是较进化类型,玉竹和长梗黄精是较原始类型。The isoenzyme pattern of ascorbate oxidase（AOD）, polyphenoloxidase （PPO）, superoxidase （SOD）, esterase（EST） ,and peroxidase（ POD） from six species of Polygonatum, originated in Dabieshan Mountain in west of Anhui Province, were compared and analyzed by PAGE. The results indicate.（1）Sixty-six isoenzyme bands in total for five kinds of isoenzymes appeared in the analysis and three bands among them are special to Polygonatum. As to a definite isoenzyme,the isoenzyme number,isoenzyme Rf and isoenzyme activity are different for each species, showing great diversity. （2）The result by Fuzzy cluster analysis is coincidence with morphological taxonomy, and the differences of isozyme bands can be used to identify the six species from each other primarily. （3） Among six species of Polygonatum, P. zanlanscianense, P. verticillatum, P. cyrtonema and P. jinzhaiense are more evolutive than P. odoratum and P. zanlanscianense.

关 键 词：黄精属 同工酶 模糊聚类分析 特征酶带 

分 类 号：Q945[生物学—植物学] Q55