应用ISSR标记研究仲彬草属植物的遗传变异  

Study on genetic variation of genus Kengyilia by ISSR markers

作　　者：张利[1] 周永红[1] 丁春邦[1] 杨瑞武[1] 刘世贵[2] 

机构地区：[1]四川农业大学小麦研究所,四川都江堰611830 [2]四川大学草原生防国家实验室,四川成都610064

出　　处：《广西植物》2006年第4期375-380,394,共7页Guihaia

基　　金：国家自然科学基金(30270099;30470135);四川省教育厅;科技厅重点项目~~

摘　　要：利用ISSR标记对仲彬草属14个种和1个变种共32份材料进行了研究。结果表明：仲彬草属材料间ISSR标记多态性较高，变异较大。12个引物共扩增出593条带，其中535条（90．2％）具有多态性，每个引物可扩增出11～80条多态性带，平均44．6条。ISSR标记遗传相似系数在种间变化范围为0．430～0．866，平均值为0．620。从聚类分析得知，ISSR标记能将32份仲彬草属材料完全分开，32份材料聚为4类。同种不同居群的材料分别聚在一起，亲缘关系较近，同时，在分子水平上种内不同居群间也存在分化；种间存在明显的遗传差异；形态相似、地理分布一致的物种倾向于聚类在一起，有一定的亲缘关系。因此，ISSR分子标记能有效地评价仲彬草属物种的亲缘关系。The results of ISSR markers showed that higher genetic variation was present among the materials analyzed. A total of 593 bands were amplified from 12 primers,of which 535 bands（90. 2%）were polymorphic. 11-80 polymorphic bands could be amplified from each primer, with an average of 44.6 bands. The average GS value of species was 0. 620 , ranging from 0. 430 to 0. 866. Cluster analysis showed that all materials could be distinguished by ISSR markers and classified into 4 groups. The different accessions in a species were clustered together respectively,and had genetic variation in molecular levels. Distinct genetic differences exist among the species;The species with similar morphological characters and the species from the same areas or neighboring geographical regions were clustered together,and had close relationships. It is a useful method for analysis of the genetic variation in Kengyilia.

关 键 词：仲彬草属 ISSR标记 遗传变异 亲缘关系 

分 类 号：Q943[生物学—植物学]