紫杉醇提取纯化工艺  

IMPROVED PURIFICATION OF TAXOL

作　　者：阎家麒[1] 范金城[1] 王九一[1] 

机构地区：[1]河南绿十字生物工程研究所,河南省分析测试中心

出　　处：《中国医药工业杂志》1996年第12期531-533,共3页Chinese Journal of Pharmaceuticals

摘　　要：以云南红豆杉树皮（或树叶）为原料，提取纯化紫杉醇。先经９５％乙醇、二氯甲烷提取，再经硅藻土柱涂渍色谱、硅胶６０中压快速柱色谱分离，收集含紫杉醇－ｃｅｐｈａｌｏｍａｎｎｉｎｅ的组分后以Ｃ_（１８）制备型ＲＰ－ＨＰＬＣ分离，两次结晶后得到紫杉醇无色结晶固体，纯度≥９８．８％，收率为树皮干重的０．０２８％，树叶干重的０．００８８％。An improved method for the isolation and purification of Taxol from Taxus Yunnanensis is described. The bark(or needles) is extracted with 95% ethanol, the extract is partitioned between methylene chloride. The methylene chloride extract is dissolved in an ethyl acetate/methanol, and chromatographed on Celite 545 flash column, eluted with hexane, methylene chloride.Then, it is chromatographed on silica gel 60 medium-pressure flash column,the mobile Phase is gradually increased hexane/acetone. And it is further fractioned on a silica gel 60 medium pressure flash column, the mobile phase is methanol/ methylene chloride. Taxol was separated from cephalomannine by reversed-Phase C_(18) Preparative HPLC. The purity of product was≥98.8%, the yield(dry base) of taxol was 0.028% of the bark, and 0.0088% of the needles.

关 键 词：药物 紫杉醇 提取 纯化 

分 类 号：TQ463.23[化学工程—制药化工]