沙利度胺对小鼠皮肤移植的影响  

Effect of Thalidomide on Skin Allograft in Mice

作　　者：曾宁[1] 唐莉[1] 袁劲[1] 吴柯[1] 周洁[1] 胡涛[1] 陈忠华 

机构地区：[1]华中科技大学同济医学院附属同济医院器官移植研究所教育部卫生部重点实验室,武汉430030

出　　处：《医药导报》2006年第8期725-728,共4页Herald of Medicine

基　　金：国家重点基础研究发展计划(973计划)资助项目(课题名称:CGD临床预警指标;系统建立及其应用;基金编号:2003CB51550)

摘　　要：目的观察沙利度胺对小鼠皮肤移植物存活及生长情况的影响,探讨沙利度胺在抗同种异体排斥反应中的作用。方法建立Balb/c小鼠到C57BL/6小鼠的皮肤移植模型,将模型小鼠随机分为3组,每组12只,对照组腹腔注射吐温-80溶液+0.9%氯化钠注射液(1∶999),200 mL.kg-1.d-1;环孢素A组腹腔注射环孢素A,10 mg.kg-1.d-1;沙利度胺组腹腔注射沙利度胺,200 mg.kg-1.d-1。分别于实验第7,9,11天取小鼠皮肤移植物进行病理检测,观察各组移植物平均存活时间。结果对照组小鼠移植物平均存活时间为8.2 d,环孢素A组平均为13.5 d,沙利度胺组平均为13.2 d,环孢素A组与沙利度胺组均明显高于对照组(P<0.05),环孢素A组与沙利度胺组之间差异无显著性(均P>0.05)。第7,9,11天各组移植皮片病理检测结果显示,随着时间的延长,各组均可见移植皮下淋巴细胞浸润,腺体及毛细血管破坏逐渐加重;其中对照组皮下组织破坏严重,沙利度胺组皮下组织破坏明显减轻,环孢素A组皮下组织破坏最轻。结论沙利度胺能延长Balb/c小鼠到C57BL/6小鼠皮肤移植物的存活时间,具有一定的临床应用前景。Objective To survey the effect of thalidomide on the survival of allograft skin in mice so as to probe into the role of the drug in resisting allograft rejection. Methods The skin transplantation model was set up by grafting skin flaps from Balb/c to C57BL/6 mice. 36 mice with the skin allograft model were randomly divided into 3 equal groups：① the control group,②cyclosporin（ Cy A） group and ③ thalidomide （Tdm） group. Mice of group ① were given each an IP injection of 200 mL^-1· kg^-1 d^-1 of 0.9% sodium chloride solution plus Tween 80（1：999）. Mice of group ② were given each an IP injection of 10 mg·kg^-1· d^-1 of Cy-A while those of group ③ were given each an IP injection of 200 mg·kg^-1 ·d^-1 of Tdm. A part of mice of the 3 groups was sacrificed on day 7,9 and 11 of the experiment separately and their skin grafts were taken for pathological examination. The average survival times of the allograft in the remaining mice of the different groups were noted down. Results The average survival times of the allografts in mice of the control group, Cy A group and Tdm group were 8.2 days, 13.5 days and 13.2 days, respectively. Both of the average survival times of the allografts in mice of the Cy A group and Tdm group were significantly longer than that in mice of the control group（ P〈0.05, P〈0.05 ）. The difference between the average graft survival times in mice of the Cy A group and Tdm group, however, was insignificant （P〉0.05）. Pathologic examination of the skin grafts taken from mice sacrificed on day 7,9, and 11 of the experiment revealed a gradual aggravation of subcutaneous lmphocytic infiltration and destruction of glands and capillaries in animals of all 3 groups along with the prolongation of time. However, the destructive changes in the subcutaneous tissue was most serious in grafts from mice of the control group. The destructive changes were far less severe in skin grafts from mice of the Tdm group while those in skin grafts from mice of the Cy .A group were th

关 键 词：沙利度胺 移植 排斥反应 

分 类 号：R979.5[医药卫生—药品]