高效液相色谱法同时测定复方呋喃西林滴鼻液中盐酸麻黄碱与呋喃西林的含量  

Simultaneous Determination of Ephedrine Hydrochloride and Furacilin in Compound Furacilin Nose Drops with HPLC

作　　者：刘新云[1] 甘春英[1] 李志荣[1] 阎文强[1] 

机构地区：[1]郧阳医学院附属太和医院药剂科,湖北十堰442000

出　　处：《医药导报》2006年第8期821-822,共2页Herald of Medicine

摘　　要：目的建立同时测定复方呋喃西林滴鼻液中盐酸麻黄碱与呋喃西林含量的高效液相色谱法。方法固定相：Hypersil-C18不锈钢柱（150mm×4．6mm，5μm）；流动相：甲醇旬．02mol·L^-1磷酸二氢氨缓冲液[用磷酸和氨水调节pH值至（3．5±0．1）]（45：55）；流速：1mL·min^-1；检测波长：260nm。结果盐酸麻黄碱在40-600mg·L^-1,呋喃西林在0．8-12．0mg·L^-1范围内线性关系良好，盐酸麻黄碱的平均回收率为99．98％，RSD=0．15％；呋喃西林的平均回收率为99．95％，RSD=0、14％；重复性良好。结论该法简便、准确，可以用于该制剂的质量控制。Objective To set up an HPLC method for the simultaneous determination of ephedrine hydrochloride and furacilin in compound furacilin nose drops. Methods Stationary phase： Hypersil-C18 stainless steel column（ 150 mm × 4.5 mm,5μm） ;mobile phase： methanol -0.02 mol·L^-1ammonium dihydrogen phosphate buffer solution [ pH adjusted to （3.5±0. 1 ） with H3PO4 and ammonia solution ] （45 ： 55 ） ; flow rate ： 1 mL ·min^-1 ; detection wavelength ： 260 nm. Results Good linear relationships were shown when the concentrations of ephedrine hydroehloride and faracillin were within the range of 40 - 600 mg·L^-1 and 0.8 - 2.0 mg·L^-1 , respectively. The average rates of recovery of ephedrine hydrochloride and furacilin were 99.98%, RSD = 0.15% and 99.95%, RSD = 0.14%, respectively. The replication of the method was good. Condusion The method was shown to be handy, accurate and may be used in the quality control of the preparation.

关 键 词：呋喃西林滴鼻液 复方 麻黄碱 盐酸 呋喃西林 高效液相色谱法 含量测定 

分 类 号：R927.11[医药卫生—药学]