铅中毒幼鼠血铅、锌、铜、铁、钙水平变化  

Changes of Lead,Zinc,Copper,Iron and Calcium in Blood of Lead Poisoned Infantal Mice

作　　者：何瑞芳[1] 张艳[2] 杨艳旭[3] 李晓娟[1] 程远[1] 李东亮[1] 

机构地区：[1]新乡医学院生理学与神经生物学教研室,河南新乡453003 [2]新乡医学院机能学研究室,河南新乡453003 [3]新乡医学院公共卫生学系,河南新乡453003

出　　处：《实用儿科临床杂志》2006年第14期936-937,共2页Journal of Applied Clinical Pediatrics

基　　金：河南省自然科学基金项目资助(0111020300);新乡医学院神经生物学学科经费资助

摘　　要：目的探讨慢性铅中毒幼鼠血铅、锌、铜、铁、钙水平变化。方法选取健康21 d断乳昆明小鼠48只,随机分为4组,每组12只。蒸馏水为对照组,余3组分别为10、20、40 mg/kg醋酸铅染毒组,按10 mL/kg灌胃染毒,1次/d,连续染毒46 d后摘眼球采血,用BS微量元素分析仪对血铅、锌、铜、铁水平进行测定,血浆钙用Dimension-RXL全自动生化分析仪测定。结果随着给铅水平的增加,小鼠血铅和铜均升高,而锌下降,铅中毒组与对照组相比,血铅、锌、铜均有显著性差异(P<0.01,0.05),而全血铁和血浆钙水平均无显著性差异(P均>0.05)。结论铅中毒可引起血锌下降和血铜升高,锌可能具有拮抗铅的毒性作用。实用儿科临床杂志,2006,21(14)Objective To investigate the changes of lead,zinc, copper, iron and calcium in blood of chronic poisoned infantal mice. Methods Forty eight 21 day old kunzea mice were randomly divided into 4 groups,each having 12 mice. Distilled water group was as control group and other three lead acetate poisoning groups had a dose of 10,20,40 mg/kg, respectively. The poisoning was carried out by lavage once a day, and consecutively for 46 days. Eyeballs of mice were picked then for blood .sampling, and BS, trace element analysis grapher was used to determine level of lead,zinc,copper and iron. Level of calcium was measured by Dimentional-RXL auto- biochemistry analysis meter. Results The lead and zinc levels in poisoned mice blood were increased with increasing lead acetate level administration, while zinc level changed inversely with lead acetate level. Significant differences were shown among control group and poisoning groups in terms of lead （ P〈0.01 ） ,zinc（ P〈0.01 ）and copper （ P〈 0.05）. There were no remarkable concentration changes of calcium and iron between control group and poisoning groups（all P 〉0.05 ） . Conclusion Lead posioning can lead to zinc decreasing and copper increasing, which suggests that zinc works as a pocntial antidote of lead poisoning.

关 键 词：鼠 铅中毒 铅 锌 铜 铁 钙 

分 类 号：R725.9[医药卫生—儿科]