三疣梭子蟹消化酶的初步研究  

Studies on Digestive Enzyme Activities of Portunus trituberculatus

作　　者：胡毅[1] 潘鲁青[1] 

机构地区：[1]中国海洋大学教育部海水养殖重点实验室,山东青岛266003

出　　处：《中国海洋大学学报（自然科学版）》2006年第4期621-626,共6页Periodical of Ocean University of China

基　　金：国家自然科学基金项目(30100140);高等学校博士学科点专项科研基金项目(2000042303)资助

摘　　要：开发研制配合饲料，以酶学分析方法研究了温度、pH对三疣梭子蟹（Portunus trituberculatus）中肠腺3种主要消化酶（蛋白酶、淀粉酶和纤维素酶）活性的影响及消化酶（蛋白酶、胰蛋白酶、胰凝乳蛋白酶、羧肽酶A、羧肽酶B、淀粉酶、脂肪酶和纤维素酶）在胃、中肠腺、肠道中的活性分布。结果表明：蛋白酶、淀粉酶、纤维素酶的最适温度分别为55℃，55℃，65℃；蛋白酶、淀粉酶、纤维素酶的最适pH分别为7～8，7．5。4．8；蛋白酶、淀粉酶、纤维素酶在短时间内有很高的热稳定性及很广的酸碱稳定性范围。蛋白酶、胰蛋白酶、羧肽酶A、羧肽酶B活性均以中肠腺最高，胃和肠道中没有测出胰凝乳蛋白酶活性；淀粉酶活性在胃、中肠腺、肠道中差别不大；纤维素酶和脂肪酶活性在胃和肠道高于中肠腺。The effects of temperature and pH on the activities of protease, amylase and cellulase of Portunus trituberculatus midgut gland and the digestive enzymes （protease, trypsin, chymotry psin, carboxypeptidase A, carboxypeptidase B, amylase, cellulase and liapse） from different digestive tissues of Portunus tritubercula- tus were studied by using enzyme analytical method. The results indicated that： the optimal temperatures were 55℃ for protease and amylase and 65℃ for cellulase, the optimal pH values were 7 - 8 for protease, 7.5 for amylase, and 4.8 for cellulase. The protease, amylase, and cellulase from Portunus trituberculatus midgut gland had high thermostability and pH-stability in 2h and 4 h. The protease, trypsin, carboxypeptidase A and carboxypeptidase B had higher activity in the midgut gland than the stomach and intestine, and the chymotrypsin activity had not been found in the stomach and intestine. Amylase had the same activity in the midgut gland, the stomach and intestine, lipase and cellulase had higher activity in the stomach and intestine than the midgut gland.

关 键 词：三疣梭子蟹 温度 PH 消化酶 热稳定性 酸碱稳定性 

分 类 号：S917[农业科学—水产科学]