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作 者:缴丽莉[1] 倪志云[2] 路丙社[1] 白志英[1] 周如久[1] 冯蕾[3]
机构地区:[1]河北农业大学园林与旅游学院,河北保定071000 [2]河北省林业局,河北石家庄050000 [3]衡水学院生物系,河北衡水053000
出 处:《河北农业大学学报》2006年第4期44-47,共4页Journal of Hebei Agricultural University
摘 要:以3年生青榨槭幼树1年生枝条为材料,测定了不同冷冻处理条件下细胞超氧化物歧化酶(SOD)和过氧化物酶(POD)活性、丙二醛(MDA)含量以及枝条的相对电导率。结果表明:随着温度下降,细胞SOD和POD酶活性均呈现先升高后下降的趋势,MDA含量随温度降低逐渐升高;枝条相对电导率随处理温度降低呈“S”形上升。相对电导率结合Logistic方程推算的青榨槭低温半致死温度(LT50)为-29.93℃,与枝条恢复生长试验结果基本一致,因此,相对电导率可以作为测定青榨槭低温半致死温度简洁、有效的生理指标。Under the low temperature treatments, the activities of SOD and POD, the content of Malondialdehyde (MDA) and the relative electrolyte leakage percentage of David maple's (Acer davidii ) sticks were studied in the paper. It showed that the activities of SOD and POD in membrane increased early and decreased later; the content of MDA increased, and the relative electric conductivity increased as a S- curves with the temperature decreased. According to the Logistic equation, we could calculate the semi - lethal temperature ( LT50 ) for david maple' s one - year stick was -29.93℃, which is in agreement with the recovery test result, which may be an important index of cold resistance in David maple.
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