长期放置含铜IUD与ras、p53基因突变及HPV感染的关系  

Relationship between Long-term Placement of IUD and Ras p53 Gene Mutations or HPV Infection

作　　者：张萍[1] 董白桦[2] 张爱荣[1] 高晓林[1] 孔北华[2] 

机构地区：[1]山东大学第二医院妇产科,济南250033 [2]山东大学齐鲁医院妇产科,济南250012

出　　处：《生殖与避孕》2006年第7期414-417,共4页Reproduction and Contraception

摘　　要：目的:探讨长期(5年以上)放置含铜宫内节育器(intrauterinedevice,IUD)与宫颈细胞ras、p53基因突变及人乳头瘤病毒(Humanpapillomaviruses,HPV)感染的关系。方法:采集宫颈脱落细胞学液基标本,采用伯塞斯达系统(thebethesdasystem,TBS)诊断标准行宫颈细胞病理学诊断;采用PCR-SSCP技术检测标本中H-ras、p53基因的表达;采用FQ-PCR技术检测肿瘤相关HPVDNA含量。结果:H-ras及p53基因表达在各组中均无改变;含铜IUD组中HPV6/11型阳性率为3.33%,DNA含量为8.80×107copy/ml,对照组中HPV6/11型阳性率为3.70%,DNA含量为6.75×107copy/ml,两组相比差异无统计学意义(P>0.05)。两组HPV16/18型均为阴性。结论:长期放置含铜IUD(带尾丝)对子宫颈无致突变作用,不增加肿瘤相关HPV感染,不增加子宫颈病变的发生率。Objective： To study the relationship between copper-releasing tailed Intrauterine devices（IUD） and ras p53 gene mutations or human papillomavirus （HPV） infection. Methods： PCR-SSCP technique was used to determine the expression of gene H-ras and p53 in cervical scraping ThinPrep samples,The fixed quantity of HPV DNA was detected by fluorescence quantitative-PCR（FQ-PCR） method. Results： There was no positive expression of gene H-ras and p53 in all cervical scraping samples.The positive rate of HPV6/11 was 3.33% and the fixed quantity of HPV6/11 DNA was 8.80 ×10^7 copy/ml in copper-releasing tailed IUD group,In control group the positive rate and the quantity of HPV6/11 were 3,70% and 6.75 ×10^7 copy/ml, respectively.There was no significant difference between the two groups. HPV16/18 of all samples were negative. Conclusion： The risk of cervix diseases was not increased for women using copper-releasing tailed IUD for long term.

关 键 词：宫内节育器 H-RAS基因 P53基因 HPV DNA定量 人乳头瘤病毒 

分 类 号：R169.42[医药卫生—公共卫生与预防医学] R711.7