PKC、PKB通过p21^(CIP1/WAF1)蛋白对小鼠受精卵G2期向M期转换的影响  

Effect of Protein Kinase C and Protein Kinase B on G2/M Transition by Affecting p21 in Mouse Fertilized Eggs

作　　者：武迪迪[1] 具英花[1] 张杰[1] 李雪松[1] 于爱鸣[1] 宗志宏[1] 安丽文[1] 于秉治[1] 

机构地区：[1]中国医科大学基础医学院生物化学与分子生物学教研室,沈阳110001

出　　处：《生殖与避孕》2006年第7期387-391,432,共6页Reproduction and Contraception

基　　金：国家重点基础研究发展规划项目(973)(G1999055900-2);国家自然科学基金重点项目(39730460)

摘　　要：目的:探讨在小鼠受精卵中,PKC及PKB是如何通过p21CIP1/WAF1蛋白影响小鼠受精卵G2/M的转换。方法:以小鼠受精卵为材料,通过Westernblot及免疫荧光的方法,检测经PKC及PKB抑制剂处理后的p21蛋白表达及定位。结果:经PKC抑制剂(PMA)处理后的小鼠G2期受精卵中p21蛋白表达增加,而经PKB抑制剂(LY294002)处理后的p21蛋白表达没有变化,但是其在亚细胞的定位发生了改变。结论:PKC通过改变p21蛋白表达,影响小鼠受精卵G2/M转换。而PKB通过改变p21蛋白在小鼠受精卵中的亚细胞定位,影响受精卵G2/M转换。Objective： To explore effect of PKC and PKB on G2/M Transition by Affecting p21 in mouse fertilized eggs. Methods： The expression and subcellular localization of p21 in fertilized eggs were detected by Western blotting and immunocytochemistry after treated with PKB and PKC inhibitors. Results： PKC inhibitor induce upregulation of p21 protein in mouse fertilized eggs in G2 phase, while PKB had no effect on it. p21 protein in G2 phase was located in the cytoplasm and nucleus, redistributing in the nucleus treated with PKB inhibitor. Conclusion： The PKC controlled G2/M transition by affecting the expression of p21 protein while PKB controlled G2/M transition by changing the subcellular localization of p21 protein.

关 键 词：PKC PKB P21蛋白 小鼠G2期受精卵 G2/M转换 

分 类 号：Q954.4[生物学—动物学]