白念珠菌的毒力及其对氟康唑耐药性的相关性研究  

Relationship between virulence of Candida albicans and its resistance to fluconazole

作　　者：叶夏云[1] 敖燕[1] 钟建庭[1] 刘丽[1] 刘冬梅[1] 

机构地区：[1]兰州军区总医院普内皮肤科,兰州730050

出　　处：《中国麻风皮肤病杂志》2006年第7期535-537,共3页China Journal of Leprosy and Skin Diseases

摘　　要：目的:研究白念珠菌的毒力因子分泌性蛋白酶(SAP)活力与其对氟康唑耐药性形成之间的关系。方法:(1)将对氟康唑敏感的白念珠菌,培养在含氟康唑浓度逐渐增加的液体培养基中,体外诱导为氟康唑耐药株;(2)运用牛血清白蛋白培养基方法,分别测定了对氟康唑敏感和对氟康唑耐药的白念珠菌的分泌性蛋白酶活力。结果:(1)40株对氟康唑敏感的白念珠菌均在体外被成功地诱导为对氟康唑的耐药株;(2)耐药株的分泌性蛋白酶活力明显强于敏感株(P<0.001)。结论:白念珠菌的毒力因子分泌性蛋白酶活力增加与其对氟康唑耐药性的增加相一致。Objective： To determine the relationship between virulence production of secreted aspartyl proteinase （SAP） of Candida albicans and the development of the resistance to fluconazole. Methods： （1） Fluconazole- susceptible Candida albicans isolates were cultured in liquid medium in which fluconazole concentration increased from 8.0 to 128 μg/mL, experimentally to induce fluconazole resistance. （2） The secretory proteinase activity （SAP） of fluconazole - susceptible Candida albicans isolate and fluconazole- resistant Candida albicans isolate were measured by bovine serum albumin assay. Results： （1） Forty fluconazole - susceptible Candida albicans isolates were in vitro induced into fluconazole- resistant Candida albicans isolates. （2） The SAP activity of fluconazole- resistant Candida albicans strains was stronger than that of fluconazole - susceptible Candida albicans isolates （ P 〈 0. 001 ）. Conclusion： Enhanced SAP production of Candida albicans isolates is correlated to the develolanent of resistance to fluconazole.

关 键 词：白念珠菌 氟康唑 耐药 分泌性蛋白酶 

分 类 号：R379[医药卫生—病原生物学]