成年大鼠脊髓损伤后血-脊髓屏障通透性变化  

THE CHANGES OF BLOOD-SPINAL CORD BARRIER PERMEABILITY AFTER SPINAL CORD INJURY IN RATS

作　　者：赵宇[1] 沈学锋[1] 程希平[1] 焦西英[1] 游思维[1] 鞠躬[1] 

机构地区：[1]第四军医大学基础部全军神经科学研究所,西安710032

出　　处：《神经解剖学杂志》2006年第4期437-440,共4页Chinese Journal of Neuroanatomy

基　　金：国家重点基础研究计划(2003CB515301);国家自然科学基金(No.30371466)资助项目

摘　　要：为了观察脊髓挤压伤后血-脊髓屏障（BSCB）的通透性变化，本研究选用体重180～200g成年雄性SD大鼠39只，随机分为对照组（n=3）和脊髓损伤组（n=6）。后者又分为伤后0、8、24、72h以及1周、2周等6组，每组6只，再分为A组、B组，每组各3只。脊髓损伤组A组的组织切片行H．E．染色，显微镜下观察脊髓损伤后的形态学变化。对照组和B组动物股静脉注射30g／L伊文思蓝，30min后，以温生理盐水及4％多聚甲醛灌注动物，取出脊髓，行冰冻切片，荧光显微镜下观察。结果观察到，脊髓挤压伤术后72h内，损伤区周围均可见伊文思蓝染色，至1周后损伤区周围未见伊文思蓝染色。本研究结果提示，脊髓损伤72h内可能为有效给药时间窗，1周后给药，药物对脊髓损伤的治疗作用将难以达到治疗目的。To observe the changes of blood-spinal cord harrier（BSCB） permeability after compressive spinal, cord injury, thirty-nine male adult Sprague-Dawley rats（ 180 - 200 g） were used in this study. There were one control group of 3 rats and six spinal cord injury groups of 36 rats which were assigned to subgroups of O h, 8 h, 24 h, 72 h, 1 w and 2 w （the time of post-injury）. Spinal cord injury groups were further assigned to A and B groups, each of which contained 3 rats. H.E. staining was used in A group to observe morphologic changes of spinal cord after injury by microscope. Evans blue（30 g/L） was injected into rats of the control group and B group through the femoral vein, and then used warm saline and 4% paraformaldehyde to perfuse these rats 30 minutes later. Spinal cord was taken out and cryosectioned immediately for histological examination by a fluorescence mieroscope. We observed that the red fluorescence of Evans blue was seen around the lesion site within 72 h after spinal cord injury, but was not seen in spinal cord 1 week later after injury. The present results suggest that the effective time of drugs administration is possiblely within 72 h after spinal cord injury. It will be hard for drags to act on the injury if administrating drags one week later.

关 键 词：脊髓损伤 血-脊髓屏障 伊文思蓝 大鼠 

分 类 号：R651.2[医药卫生—外科学]